Cerberus 1/CER1 Protein, Human (HEK293, His)
Based on 1 publication(s) in Google Scholar
The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Cerberus 1, DAN family BMP antagonist (CER1), is a cysteine knot superfamily protein homolog and a DAN family BMP antagonist. CER1 has nine conserved cysteines and a cysteine knot region, together with Dan and DRM/Gremlin represent bone morphogenetic protein (BMP) antagonists that can directly bind to BMP and inhibit its activity. Upregulation of CER1 in human ES cells leads to inhibition of Nodal signaling associated with human ES cell differentiation. CER1 (Cerberus 1) and GREM3 (CKTSF1B3 or CER2) inhibit NODAL signaling through ACVR1B (ALK4) or ACVR1C (ALK7) to SMAD2 or SMAD3. The network formed by the Nodal and BMP signaling pathways and the WNT signaling pathway is related to embryogenesis and carcinogenesis. Although, CER1 inhibits Wnt3a-induced classical Top Flash reporter to some extent, it does not affect Wnt11 signaling in cell culture. Furthermore, CER1 fine-tunes the spatial organization of the ureteral tree by coordinating the activities of the growth-promoting ureteric bud signals Gndf and Wnt11 through Bmp-mediated antagonism and, to some extent, canonical Wnt signaling involved in branching.
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
AAI03977.1 (T18-A267)
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Molecular Construction
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N-term
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CER1 (T18-A267)
Accession # AAI03977.1 -
6*His
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C-term
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Protein Length
Full Length of Cerberus Chain
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Synonyms
CER1; DAN Domain Family Member 4; Cerberus 1, DAN Family BMP Antagonist; Cerberus 1 (Xenopus Laevis) Homolog (Cysteine Knot Superfamily); DAND4; Cerberus 1, Cysteine Knot Superfamily, Homolog (Xenopus Laevis); Cerberus-Related Protein; Cerberus 1, Cystein
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AA Sequence
TRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKLVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA
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Predicted Molecular Mass
29.2 kDa
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Molecular Weight
Approximately 35-42 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM NaAc-HAC, pH 4.5.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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