2. Recombinant Proteins
  3. Receptor Proteins
  4. G-Protein-Coupled Receptors (GPCRs)
  5. Muscarinic Acetylcholine Receptor
  6. Cholinergic Receptor Muscarinic 1 (CHRM1)
  7. CHRM1 Protein, Rat (His)

CHRM1 Protein, Rat (His)

Cat. No.: HY-P72138
Handling Instructions

CHRM1, the muscarinic acetylcholine receptor, mediates cellular responses, including adenylate cyclase inhibition, phosphoinositide breakdown, and potassium channel modulation through G protein actions. It engages with GPRASP2 and TMEM147, contributing to a complex network of signaling pathways and cellular processes regulated by the receptor. CHRM1 Protein, Rat (His) is the recombinant rat-derived CHRM1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CHRM1 Protein, Rat (His) is 157 a.a., with molecular weight of ~21.1 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CHRM1, the muscarinic acetylcholine receptor, mediates cellular responses, including adenylate cyclase inhibition, phosphoinositide breakdown, and potassium channel modulation through G protein actions. It engages with GPRASP2 and TMEM147, contributing to a complex network of signaling pathways and cellular processes regulated by the receptor. CHRM1 Protein, Rat (His) is the recombinant rat-derived CHRM1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CHRM1 Protein, Rat (His) is 157 a.a., with molecular weight of ~21.1 kDa.
Background

The muscarinic acetylcholine receptor, represented by CHRM1, is responsible for mediating diverse cellular responses, such as the inhibition of adenylate cyclase, phosphoinositide breakdown, and modulation of potassium channels through G protein-mediated actions. Its primary transducing effect involves Pi turnover. Additionally, CHRM1 interacts with GPRASP2 and TMEM147, contributing to the intricate network of signaling pathways and cellular processes regulated by this receptor.
Species

Rat
Source

E. coli
Tag

N-6*His
Accession

P08482 (R210-T366)
Gene ID

25229  [NCBI]
Molecular Construction
N-term
6*His
CHRM1 (R210-T366)
Accession # P08482
C-term
Synonyms
Chrm1; Chrm-1Muscarinic acetylcholine receptor M1
AA Sequence

RIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDSEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKEKKAART

Molecular Weight

Approximately 21.1 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CHRM1 Protein, Rat (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CHRM1 Protein, Rat (His)
Cat. No.:
HY-P72138
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.