Copy Link Print Information

Contactin-3/CNTN3 Protein, Rat (HEK293, His)

Customer Review

Based on 1 Customer Validation

Contactin-3/CNTN3 protein mediates crucial cell surface interactions in nervous system development and promotes neurite outgrowth. It interacts with PTPRG, indicating its involvement in signaling mechanisms that regulate neuronal growth and connectivity. Contactin-3/CNTN3 Protein, Rat (HEK293, His) is the recombinant rat-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Rat (HEK293, His)
  • Contactin-3/CNTN3 Protein, Rat (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Contactin-3/CNTN3 protein mediates crucial cell surface interactions in nervous system development and promotes neurite outgrowth. It interacts with PTPRG, indicating its involvement in signaling mechanisms that regulate neuronal growth and connectivity. Contactin-3/CNTN3 Protein, Rat (HEK293, His) is the recombinant rat-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

Background

Contactin-3/CNTN3 protein is involved in mediating essential cell surface interactions crucial for nervous system development. This protein also possesses neurite outgrowth-promoting activity, indicating its role in promoting the extension of neuronal processes. Additionally, Contactin-3/CNTN3 protein interacts with PTPRG, suggesting its involvement in intricate signaling mechanisms that regulate neuronal growth and connectivity.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Rat CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.098 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Rat CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.098 μg/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q62682 (E20-G1001)

  • Gene ID
    54279  [NCBI]
  • Molecular Construction
    • N-term
    • CNTN3 (M1-G1001)
      Accession # Q62682
    • His
    • C-term
  • Protein Length

    Full Length of Contactin-3 Chain

  • Synonyms

    CNTN3; Plasmacytoma-Associated Neuronal Glycoprotein; Prev. PANG; Contactin-3; BIG-1; KIAA1496; Brain-Derived Immunoglobulin Superfamily Protein 1; PCS; Contactin 3; Contactin 3 (Plasmacytoma Associated)

  • AA Sequence

    ELLLQGPVFVKEPSNSIFPVGSEDKKITLNCEARGNPSPHYRWQLNGSDIDTSLDHRYKLNGGNLIVINPNRNWDTGSYQCFATNSLGTIVSREAKLQFAYLENFKSRMRSRVSVREGQGVVLLCGPPPHSGELSYAWVFNEYPSFVEEDSRRFVSQETGHLYIAKVEPSDVGNYTCVVTSTVTNARVLGSPTPLVLRSDGVMGEYEPKIELQFPETLPAAKGSTVKLECFALGNPVPQINWRRSDGMPFPTKIKLRKFNGVLEIPNFQQEDTGSYECIAENSRGKNVARGRLTYYAKPYWVQLLKDVETAVEDSLYWECRASGKPKPSYRWLKNGDALVLEERIQIENGALTIANLNVSDSGMFQCIAENKHGLIYSSAELKVLASAPDFSRNPMKKMIQVQVGSLVILDCKPSASPRALSFWKKGDTVVREQARISLLNDGGLKIMNVTKADAGIYTCIAENQFGKANGTTQLVVTEPTRIILAPSNMDVAVGESIILPCQVQHDPLLDIMFAWYFNGTLTDFKKDGSHFEKVGGSSSGDLMIRNIQLKHSGKYVCMVQTGVDSVSSAAELIVRGSPGPPENVKVDEITDTTAQLSWTEGTDSHSPVISYAVQARTPFSVGWQNVRTVPEAIDGKTRTATVVELNPWVEYEFRVVASNKIGGGEPSLPSEKVRTEEAAPEVAPSEVSGGGGSRSELVITWDPVPEELQNGGGFGYVVAFRPLGVTTWIQTVVTSPDNPRYVFRNESIVPFSPYEVKVGVYNNKGEGPFSPVTTVFSAEEEPTVAPSHISAHSLSSSEIEVSWNTIPWKSSNGRLLGYEVRYWNNGGEEESSSKVKVAGNQTSAVLRGLKSNLAYYTAVRAYNTAGAGPFSATVNATTKKTPPSQPPGNVVWNATDTKVLLNWEQVKALENESEVTGYKVFYRTSSQNNVQVLNTNKTSAELLLPIKEDYIIEVKATTDGGDGTSSEQIRIPRITSMDARG

  • Molecular Weight

    Approximately 120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Rat CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.098 μg/mL.

  • Contactin-3/CNTN3 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Rat (HEK293, His)
  • Contactin-3/CNTN3 Protein, Rat (HEK293, His) Product Image