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IL-15R alpha & IL-15 Protein, Mouse (HEK293, His)

3 Cited Publications
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Based on 3 publication(s) in Google Scholar

IL-15R alpha Protein, the high-affinity receptor for interleukin-15, crucially signals in cis and trans, presenting IL-15 to neighboring IL2RG-expressing cells. In neutrophils, it activates SYK kinase, essential for IL-15-induced phagocytosis in a SYK-dependent manner. IL-15R alpha forms a heterotrimer with IL2RB and IL2RG, capable of self-association. Interactions with SYK underscore its intricate involvement in cellular signaling pathways. IL-15R alpha & IL-15 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived IL-15R alpha & IL-15 protein, expressed by HEK293 , with C-His labeled tag. IL-15R alpha & IL-15 Protein, Mouse (HEK293, His), has molecular weight of ~36 kDa.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for IL-15R alpha & IL-15 Protein, Mouse (HEK293, His)
  • IL-15R alpha & IL-15 Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
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Use of MCE Products

Biological Activity

Description

IL-15R alpha Protein, the high-affinity receptor for interleukin-15, crucially signals in cis and trans, presenting IL-15 to neighboring IL2RG-expressing cells. In neutrophils, it activates SYK kinase, essential for IL-15-induced phagocytosis in a SYK-dependent manner. IL-15R alpha forms a heterotrimer with IL2RB and IL2RG, capable of self-association. Interactions with SYK underscore its intricate involvement in cellular signaling pathways. IL-15R alpha & IL-15 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived IL-15R alpha & IL-15 protein, expressed by HEK293 , with C-His labeled tag. IL-15R alpha & IL-15 Protein, Mouse (HEK293, His), has molecular weight of ~36 kDa.

Background

IL-15R alpha Protein emerges as the high-affinity receptor for interleukin-15, playing a crucial role in cellular signaling. This receptor exhibits the ability to signal both in cis and trans, where IL15R from one subset of cells presents interleukin-15 to neighboring IL2RG-expressing cells. In neutrophils, IL-15R alpha binds and activates the kinase SYK in response to interleukin-15 stimulation, and it is essential for IL-15-induced phagocytosis in a SYK-dependent manner. The interleukin-15 receptor, IL15R, forms a heterotrimer consisting of IL15RA, IL2RB, and IL2RG, with IL15RA capable of self-association (By similarity). Additionally, IL-15R alpha interacts with SYK, further highlighting its intricate involvement in cellular signaling pathways (By similarity).

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q60819-1 (G33-K205) & P48346 (G30-S162)

  • Gene ID
    16169  [NCBI]&16168  [NCBI]
  • Synonyms

    IL15RA; IL-15 Receptor Subunit Alpha; Interleukin 15 Receptor Subunit Alpha; IL15RA Protein; Interleukin-15 Receptor Subunit Alpha; CD215 Antigen; IL-15RA; IL-15R-Alpha; CD215; Il15ra; Interleukin 15 Receptor, Alpha; IL15; Interleukin-15; Interleukin 15;

  • Predicted Molecular Mass

    14.7 kDa (IL-15) & 19.8 kDa (IL-15RA)

  • Molecular Weight

    Approximately 50-60 kDa & 19-23 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • IL-15R alpha & IL-15 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-15R alpha & IL-15 Protein, Mouse (HEK293, His)
  • IL-15R alpha & IL-15 Protein, Mouse (HEK293, His) Product Image