1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-7
  5. IL-7 Protein, Mouse

IL-7 Protein, Mouse

Cat. No.: HY-P73226
COA Handling Instructions

IL-7 protein regulates lymphocyte population and maintains lymphoid homeostasis by developing, expanding, and supporting survival of T-cells and B-cells. It interacts with IL7R and CSF2RG receptors to exert its effects. IL-7 Protein, Mouse is the recombinant mouse-derived IL-7 protein, expressed by E. coli , with tag free. The total length of IL-7 Protein, Mouse is 129 a.a., with molecular weight of ~18 kDa.

For research use only. We do not sell to patients.

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10 μg $160 In-stock
100 μg $820 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-7 protein regulates lymphocyte population and maintains lymphoid homeostasis by developing, expanding, and supporting survival of T-cells and B-cells. It interacts with IL7R and CSF2RG receptors to exert its effects. IL-7 Protein, Mouse is the recombinant mouse-derived IL-7 protein, expressed by E. coli , with tag free. The total length of IL-7 Protein, Mouse is 129 a.a., with molecular weight of ~18 kDa.

Background

The IL-7 protein is a hematopoietic cytokine that plays a vital role in the development, expansion, and survival of naive and memory T-cells and B-cells, thus regulating the population of mature lymphocytes and maintaining lymphoid homeostasis. It interacts with IL7R and CSF2RG receptors to exert its effects.

Biological Activity

1.Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL) and the ED50 is typically 0.4-5.2 ng/mL.
2.Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-4 ng/mL.

Species

Mouse

Source

E. coli

Tag

Tag Free

Accession

Q544C8 (E26-I154)

Gene ID

16196  [NCBI]

Molecular Construction
N-term
IL-7 (E26-I154)
Accession # Q544C8
C-term
Synonyms
Interleukin 7; Lymphopoietin-1; PBGF
Molecular Weight

Approximately 18 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-7 Protein, Mouse Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-7 Protein, Mouse
Cat. No.:
HY-P73226
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