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IL-5 Protein, Human (HEK293, His)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

The IL-5 Protein, predominantly expressed by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production, growth, and differentiation. IL-5 Protein activates kinases like LYN, SYK, and JAK2, which signal through RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits. IL-5 Protein, Human (HEK293, His) is the recombinant human-derived IL-5 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-5 Protein, Human (HEK293, His)
  • Experimental Validation Results for IL-5 Protein, Human (HEK293, His)
  • IL-5 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The IL-5 Protein, predominantly expressed by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production, growth, and differentiation. IL-5 Protein activates kinases like LYN, SYK, and JAK2, which signal through RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits. IL-5 Protein, Human (HEK293, His) is the recombinant human-derived IL-5 protein, expressed by HEK293 , with C-His labeled tag.

Background

IL-5 Protein is a homodimeric cytokine predominantly expressed by T-lymphocytes and NK cells, and it plays a crucial role in the survival, differentiation, and chemotaxis of eosinophils. Additionally, IL-5 Protein acts on both activated and resting B-cells, inducing immunoglobulin production, growth, and differentiation. Its biological effects are mediated through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB, leading to the activation of various kinases such as LYN, SYK, and JAK2. These activated kinases propagate signals through the RAS-MAPK and JAK-STAT5 pathways. IL-5 Protein exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits.

Verified Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.1-0.5 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 this effect is 0.2039 ng/mL, corresponding to a specific activity is 4.904×106 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P05113-1 (I20-S134)

  • Gene ID
    3567  [NCBI]
  • Molecular Construction
    • N-term
    • IL-5 (I20-S134)
      Accession # P05113-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    IL5; T-Cell Replacing Factor; Interleukin 5; EDF; Eosinophil Differentiation Factor; Colony-Stimulating Factor, Eosinophil; Interleukin-5; B-Cell Differentiation Factor I; IL-5; Interleukin 5 (Colony-Stimulating Factor, Eosinophil); TRF; B Cell Differenti

  • AA Sequence

    IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

  • Predicted Molecular Mass

    14.2 kDa

  • Molecular Weight

    Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 this effect is 0.2039 ng/mL, corresponding to a specific activity is 4.904×106 units/mg.

  • IL-5 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-5 Protein, Human (HEK293, His)
  • Experimental Validation Results for IL-5 Protein, Human (HEK293, His)
  • IL-5 Protein, Human (HEK293, His) Product Image