IL-5 Protein, Human
Based on 1 Customer Validation
IL-5 Protein, Human is a selective activator of human eosinophil function.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IL-5 Protein, Human is a selective activator of human eosinophil function.
Recombinant Human Interleukin-5 (rhIL-5) is a powerful and selective stimulator of human eosinophil function. IL-5 is thus the first hemopoietic factor whose elaboration in vivo can explain the selective eosinophilia and eosinophil activation seen in disease[1]. Recombinant human interleukin-5 (rhIL-5), in either liquid or semi- solid cultures, selectively induced eosinophil production from normal human bone marrow, with no activity on other cell lineages. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrowcultures: comparison and interaction with IL-1, IL-3, IL-6, and granulocyte-macrophage colony stimulating factor (GMCSF)[2].
The ED50 is <1.0 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >1.0 × 106 units/mg.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P05113-1 (I20-S134)
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Molecular Construction
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N-term
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IL-5 (I20-S134)
Accession # P05113-1 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IL5; T-Cell Replacing Factor; Interleukin 5; EDF; Eosinophil Differentiation Factor; Colony-Stimulating Factor, Eosinophil; Interleukin-5; B-Cell Differentiation Factor I; IL-5; Interleukin 5 (Colony-Stimulating Factor, Eosinophil); TRF; B Cell Differenti
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AA Sequence
IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES
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Molecular Weight
Approximately 27 kDa, based on SDS-PAGE under non reducing (N) conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 25 mM Tris, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<0.2 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Lopez AF, et al. Recombinant human interleukin 5 is a selective activator of human eosinophil function. J Exp Med. 1988 Jan 1;167(1):219-24. [Content Brief]
[2]. Clutterbuck EJ, et al. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrowcultures: comparison and interaction with IL-1, IL-3, IL-6, and GMCSF. Blood. 1989 May 1;73(6):1504-12. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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