Integrin alpha V beta 8 Protein, Mouse (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Verified Bioactivity
Measured by its binding ability in a functional ELISA.Immobilized Mouse Latent TGFB1, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB8 Heterodimer Proteinwith a linear range of 1-20 ng/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-Avi;C-His
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Accession
P43406 (F31-V988)&Q0VBD0 (G22-L681)
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Gene ID16410&320910
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Molecular Construction
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N-term
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ITGAV (F31-V988)
Accession # P43406 -
His-Avi
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C-term
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N-term
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ITGB8 (G22-L681)
Accession # Q0VBD0 -
C-term
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Conjugation
Biotin
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Conjugate Method
The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
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Synonyms
ITGAV; Integrin, Alpha V (Vitronectin Receptor, Alpha Polypeptide, Antigen CD51); Prev. MSK8; Vitronectin Receptor; Prev. VNRA; Integrin AlphaVbeta3; Prev. VTNR; Integrin, Alpha V; Vitronectin Receptor Subunit Alpha; CD51 Antigen; Integrin Alpha-V; IDNDC;
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Predicted Molecular Mass
114.2 kDa (ITGAV) & 77.5 kDa (ITGB8)
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Molecular Weight
Approximately 135-145 kDa (ITGAV) & 75-85 kDa (ITGB8), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (233 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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