LRRN3/NLRR-3 Protein, Human (sf9, His)
Neuronal leucine-rich repeat protein-3 (NLRR-3), also known as LRRN3, potentiates Ras-MAPK signaling by facilitating internalization of EGF in clathrin-coated vesicles. NLRR-3 also regulates cellular differentiation. LRRN3/NLRR-3 Protein, Human (sf9, His) is the recombinant human-derived LRRN3/NLRR-3 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Neuronal leucine-rich repeat protein-3 (NLRR-3), also known as LRRN3, potentiates Ras-MAPK signaling by facilitating internalization of EGF in clathrin-coated vesicles. NLRR-3 also regulates cellular differentiation[1][2]. LRRN3/NLRR-3 Protein, Human (sf9, His) is the recombinant human-derived LRRN3/NLRR-3 protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background
Neuronal leucine-rich repeat protein-3 (NLRR-3), belongs to the LRR superfamily. NLRR-3 enhances phosphorylation of MAPK when COS-7 cells is transfected with NLRR-3. Mouse NLRR-3 also potentiates Ras-MAPK signaling by facilitating internalization of EGF in clathrin-coated vesicles[1]. NLRR-3 is associated with the regulation of cellular differentiation[1]. In addition, LRRN3 is a marker of naïve T.
Human NLRR-3 shares about 87% aa sequence identity with mouse and rat. Rat NLRR-3 shares about 95% aa sequence identity with mouse.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-His
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Accession
Q9H3W5 (M1-T628)
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Molecular Construction
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N-term
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LRRN3 (M1-T628)
Accession # Q9H3W5 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
LRRN3; Leucine-Rich Repeat Neuronal Protein 3; Leucine Rich Repeat Neuronal 3; Neuronal Leucine-Rich Repeat Protein 3; FIGLER5; FLJ11129; NLRR3; NLRR-3; Fibronectin Type III, Immunoglobulin And Leucine Rich Repeat Domains 5; Leucine-Rich Repeat Protein, N
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AA Sequence
MKDMPLRIHVLLGLAITTLVQAVDKKVDCPRLCTCEIRPWFTPRSIYMEASTVDCNDLGLLTFPARLPANTQILLLQTNNIAKIEYSTDFPVNLTGLDLSQNNLSSVTNINVKKMPQLLSVYLEENKLTELPEKCLSELSNLQELYINHNLLSTISPGAFIGLHNLLRLHLNSNRLQMINSKWFDALPNLEILMIGENPIIRIKDMNFKPLINLRSLVIAGINLTEIPDNALVGLENLESISFYDNRLIKVPHVALQKVVNLKFLDLNKNPINRIRRGDFSNMLHLKELGINNMPELISIDSLAVDNLPDLRKIEATNNPRLSYIHPNAFFRLPKLESLMLNSNALSALYHGTIESLPNLKEISIHSNPIRCDCVIRWMNMNKTNIRFMEPDSLFCVDPPEFQGQNVRQVHFRDMMEICLPLIAPESFPSNLNVEAGSYVSFHCRATAEPQPEIYWITPSGQKLLPNTLTDKFYVHSEGTLDINGVTPKEGGLYTCIATNLVGADLKSVMIKVDGSFPQDNNGSLNIKIRDIQANSVLVSWKASSKILKSSVKWTAFVKTENSHAAQSARIPSDVKVYNLTHLNPSTEYKICIDIPTIYQKNRKKCVNVTTKGLHPDQKEYEKNNTTT
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Predicted Molecular Mass
70 kDa
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Molecular Weight
Approximately 70 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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