MFG-E8 Protein, Mouse (HEK293, His)

The MFG-E8 protein plays a critical role in promoting the phagocytic clearance of apoptotic cells in various tissues. As a specific ligand, it interacts with alpha-v/beta-3 and alpha-v/beta-5 receptors and also binds independently to phosphatidylserine-rich cell surfaces. MFG-E8 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MFG-E8 protein, expressed by HEK293 , with N-His labeled tag. The total length of MFG-E8 Protein, Mouse (HEK293, His) is 441 a.a., with molecular weight of 60-70 kDa.

MFG-E8 (Milk Fat Globule-Epidermal Growth Factor 8) is a versatile protein that contributes significantly to the phagocytic removal of apoptotic cells in various tissues. Serving as a specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors, MFG-E8 facilitates the recognition and clearance of apoptotic cells by phagocytes. Additionally, it exhibits binding capabilities to phosphatidylserine-enriched cell surfaces independently of receptors. With its role as a zona pellucida-binding protein, MFG-E8 may contribute to gamete interactions (By similarity). Notably, MFG-E8 is crucial for maintaining intestinal epithelial homeostasis and promoting mucosal healing, highlighting its significance in gastrointestinal health. Furthermore, MFG-E8 plays a key role in promoting VEGF-dependent neovascularization, emphasizing its involvement in angiogenesis and tissue repair processes.

Mouse

HEK293

N-His

P21956 (A23-C463)

17304  [NCBI]

60-70 kDa

Greater than 95% as determined by Tris-Bis PAGE

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.