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NEGR1 Protein, Human (HEK293, His)

Cat. No.: HY-P76508
COA Handling Instructions

The NEGR1 protein is associated with cell adhesion, suggesting a role in important cellular interactions. Its potential function as a transneural growth-promoting factor in regenerating axonal sprouting suggests a dynamic role in promoting neuronal growth. NEGR1 Protein, Human (HEK293, His) is the recombinant human-derived NEGR1 protein, expressed by HEK293 , with C-His labeled tag. The total length of NEGR1 Protein, Human (HEK293, His) is 287 a.a., with molecular weight of 45-48 KDa.

For research use only. We do not sell to patients.

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Description

The NEGR1 protein is associated with cell adhesion, suggesting a role in important cellular interactions. Its potential function as a transneural growth-promoting factor in regenerating axonal sprouting suggests a dynamic role in promoting neuronal growth. NEGR1 Protein, Human (HEK293, His) is the recombinant human-derived NEGR1 protein, expressed by HEK293 , with C-His labeled tag. The total length of NEGR1 Protein, Human (HEK293, His) is 287 a.a., with molecular weight of 45-48 KDa.

Background

NEGR1 Protein appears to be implicated in cell adhesion, suggesting a potential role in mediating interactions critical for cellular processes. Its putative function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain indicates a dynamic role in facilitating neuronal growth. The proposed similarity to other growth-promoting factors implies NEGR1's potential involvement in regenerative processes, particularly in the context of axon sprouting in the brain. Elucidating the specific mechanisms through which NEGR1 participates in cell adhesion and contributes to regenerative axon sprouting could offer valuable insights into its role in neural development and regeneration. Further exploration of NEGR1's functions may provide a better understanding of its potential implications in neuronal plasticity and regenerative processes within the mammalian brain.

Biological Activity

Measured by its ability to the proliferation of SH-SY5Y Human neuroblastoma cells. The ED50 for this effect is 14.38μg/mL, corresponding to a specific activity is 69.541 Unit/mg.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q7Z3B1-1 (V38-G324)

Gene ID
Molecular Construction
N-term
NEGR1 (V38-G324)
Accession # Q7Z3B1
His
C-term
Synonyms
Neuronal growth regulator 1; IgLON family member 4; IGLON4
AA Sequence

VDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNPPSTAQYGITG

Molecular Weight

Approximately 42-55 kDa due to the glycosylation

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NEGR1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NEGR1 Protein, Human (HEK293, His)
Cat. No.:
HY-P76508
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