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NEGR1 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

The NEGR1 protein is associated with cell adhesion, suggesting a role in important cellular interactions. Its potential function as a transneural growth-promoting factor in regenerating axonal sprouting suggests a dynamic role in promoting neuronal growth. NEGR1 Protein, Human (HEK293, His) is the recombinant human-derived NEGR1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NEGR1 Protein, Human (HEK293, His)
  • Experimental Validation Results for NEGR1 Protein, Human (HEK293, His)
  • NEGR1 Protein, Human (HEK293, His) Product Image
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609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The NEGR1 protein is associated with cell adhesion, suggesting a role in important cellular interactions. Its potential function as a transneural growth-promoting factor in regenerating axonal sprouting suggests a dynamic role in promoting neuronal growth. NEGR1 Protein, Human (HEK293, His) is the recombinant human-derived NEGR1 protein, expressed by HEK293 , with C-His labeled tag.

Background

NEGR1 Protein appears to be implicated in cell adhesion, suggesting a potential role in mediating interactions critical for cellular processes. Its putative function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain indicates a dynamic role in facilitating neuronal growth. The proposed similarity to other growth-promoting factors implies NEGR1's potential involvement in regenerative processes, particularly in the context of axon sprouting in the brain. Elucidating the specific mechanisms through which NEGR1 participates in cell adhesion and contributes to regenerative axon sprouting could offer valuable insights into its role in neural development and regeneration. Further exploration of NEGR1's functions may provide a better understanding of its potential implications in neuronal plasticity and regenerative processes within the mammalian brain.

Verified Bioactivity

Measured by its ability to the proliferation of SH-SY5Y Human neuroblastoma cells. The ED50 for this effect is 14.38μg/mL, corresponding to a specific activity is 69.541 Unit/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to the proliferation of SH-SY5Y Human neuroblastoma cells.The ED50 for this effect is 14.38 μg/mL, corresponding to a specific activity is 69.541 Unit/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q7Z3B1-1 (V38-G324)

  • Gene ID
    257194  [NCBI]
  • Molecular Construction
    • N-term
    • NEGR1 (V38-G324)
      Accession # Q7Z3B1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    NEGR1; A Kindred Of IgLON; Neuronal Growth Regulator 1; Neurotractin; IGLON4; MGC46680; IgLON Family Member 4; Alternative Protein NEGR1; KILON; DMML2433; Ntra

  • AA Sequence

    VDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNPPSTAQYGITG

  • Molecular Weight

    Approximately 45-48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to the proliferation of SH-SY5Y Human neuroblastoma cells.The ED50 for this effect is 14.38 μg/mL, corresponding to a specific activity is 69.541 Unit/mg.

  • NEGR1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for NEGR1 Protein, Human (HEK293, His)
  • Experimental Validation Results for NEGR1 Protein, Human (HEK293, His)
  • NEGR1 Protein, Human (HEK293, His) Product Image