PEPD Protein, Human

PEPD Protein, a member of the peptidase family, is a dipeptidase which hydrolyze dipeptides with proline or hydroxyproline at the carboxy terminus. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. PEPD suppression of p53 is essential for cell survival and tumor growth. PEPD Protein, Human is the recombinant human-derived PEPD protein, expressed by E. coli , with tag free. The total length of PEPD Protein, Human is 492 a.a., with molecular weight of 54-60 kDa.

PEPD, a member of the peptidase family, is a dipeptidase which hydrolyze dipeptides with proline or hydroxyproline at the carboxy terminus. The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved by PEPD. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. PEPD modulates expression of interferon α/β receptor IFNAR1. PEPD directly binds to p53 in the nucleus and cytoplasm and suppresses both transcription-dependent and transcription-independent activities of p53, which is essential for cell survival and tumor growth. In addition, PEPD stimulates proliferation and migration of fibroblasts via activation of the EGFR-downstream PI3K/Akt/mTOR signaling pathway, which also stimulates the expression of β1-integrin and IGF-1 receptors and proteins downstream to these receptors such as FAK, Grb2, and ERK1/2^[1][2][3][4].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

AAH28295.1 (A2-K493)

5184  [NCBI]

AAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFFNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

54-60 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, 10% Glycerol, pH 8.5 or 50 mM Tris-HCL, 300 mM NaCL, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.