POMGNT1 Protein, Human (HEK293, His)
The POMGNT1 protein crucially directs O-mannosyl glycosylation, which adds N-acetylglucosamine to O-linked mannose residues on glycoproteins. It catalyzes GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr on α-dystroglycan and other O-mannosylated proteins, laying the foundation for subsequent carbohydrate addition. POMGNT1 Protein, Human (HEK293, His) is the recombinant human-derived POMGNT1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The POMGNT1 protein crucially directs O-mannosyl glycosylation, which adds N-acetylglucosamine to O-linked mannose residues on glycoproteins. It catalyzes GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr on α-dystroglycan and other O-mannosylated proteins, laying the foundation for subsequent carbohydrate addition. POMGNT1 Protein, Human (HEK293, His) is the recombinant human-derived POMGNT1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
POMGNT1 Protein plays a pivotal role in O-mannosyl glycosylation by facilitating the addition of N-acetylglucosamine to O-linked mannose residues on glycoproteins. Specifically, it catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and various other O-mannosylated proteins. This enzymatic activity serves as a fundamental step, laying the groundwork for subsequent additions of carbohydrate moieties. Notably, POMGNT1 demonstrates specificity for alpha-linked terminal mannose and lacks activity corresponding to MGAT3, MGAT4, MGAT5, MGAT7, or MGAT8. The intricate glycosylation process orchestrated by POMGNT1 highlights its essential contribution to the structural complexity and functional diversity of glycoproteins.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q8WZA1-1 (L59-T660)
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Molecular Construction
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N-term
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POMGNT1 (L59-T660)
Accession # Q8WZA1-1 -
6*His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
POMGNT1; Protein O-Linked-Mannose Beta-1,2-N-Acetylglucosaminyltransferase; Prev. MEB; Protein O-Mannose Beta-1,2-N-Acetylglucosaminyltransferase; MGAT1.2; Uncharacterized Protein DKFZp761B182; Protein O-Linked-Mannose Beta-1,2-N-Acetylglucosaminyltransfe
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AA Sequence
LDTRRAISEANEDPEPEQDYDEALGRLEPPRRRGSGPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGGPVFGEKHSKSPALSSWGDPVLLKTDVPLSSAEEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLGWVLRRSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEVHRLLSEAEVLDHSKNPCEDSFLPDTEGHTYVAFIRMEKDDDFTTWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLMVGVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAPEQT
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Predicted Molecular Mass
69.3 kDa
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Molecular Weight
Approximately 79 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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