PPM1H protein, Human (HEK293, His)
Based on 1 Customer Validation
PPM1H, the enzyme subject, dephosphorylates CDKN1B at 'Thr-187,' critically regulating cellular processes. By preventing proteasomal degradation, PPM1H stabilizes CDKN1B, emphasizing its role in protein stabilization. This dephosphorylation event, orchestrated by PPM1H, intricately controls CDKN1B levels, showcasing its involvement in modulating cell cycle progression and regulating key cellular functions. PPM1H, Human (HEK293, His) is the recombinant human-derived PPM1H, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PPM1H, the enzyme subject, dephosphorylates CDKN1B at 'Thr-187,' critically regulating cellular processes. By preventing proteasomal degradation, PPM1H stabilizes CDKN1B, emphasizing its role in protein stabilization. This dephosphorylation event, orchestrated by PPM1H, intricately controls CDKN1B levels, showcasing its involvement in modulating cell cycle progression and regulating key cellular functions. PPM1H, Human (HEK293, His) is the recombinant human-derived PPM1H, expressed by HEK293 , with C-His labeled tag.
The subject, PPM1H, functions as an enzyme that dephosphorylates CDKN1B at 'Thr-187,' thereby playing a critical role in the regulation of cellular processes. By removing the phosphorylation signal at this specific site, PPM1H prevents the proteasomal degradation of CDKN1B, emphasizing its role in stabilizing the protein. This dephosphorylation event orchestrated by PPM1H contributes to the intricate control of CDKN1B levels, highlighting the enzyme's involvement in the modulation of cell cycle progression and the regulation of key cellular functions.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q9ULR3 (M1-S514)
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Gene ID57460
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Molecular Construction
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N-term
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PPM1H (M1-S514)
Accession # Q9ULR3 -
His
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C-term
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Protein Length
Full Length
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Synonyms
PPM1H; Protein Phosphatase 1H (PP2C Domain Containing); Prev. ARHCL1; Protein Phosphatase 1H; KIAA1157; FLJ13253; NERPP-2C; URCC2; Neurite Extension-Related Protein Phosphatase Related To PP2C; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1H; Protein Phospha
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AA Sequence
MLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS
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Predicted Molecular Mass
56.4 kDa
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Molecular Weight
Approximately 75 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 150 mM NaCl, pH 7.4, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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