Transcription termination factor Rho Protein, E.coli (His)
Based on 1 Customer Validation
The transcription terminator Rho protein plays a key role in promoting transcription termination through a mechanism that binds to nascent RNA, activates Rho's RNA-dependent ATPase activity, and subsequently releases mRNA from the DNA template. As an RNA-dependent NTPase, Rho utilizes all four ribonucleoside triphosphates as substrates, highlighting its versatility in nucleotide interactions during termination. Transcription termination factor Rho Protein, E.coli (His) is the recombinant E. coli-derived Transcription termination factor Rho protein, expressed by E. coli , with N-6*His labeled tag.
- Species: E.coli
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
The transcription terminator Rho protein plays a key role in promoting transcription termination through a mechanism that binds to nascent RNA, activates Rho's RNA-dependent ATPase activity, and subsequently releases mRNA from the DNA template. As an RNA-dependent NTPase, Rho utilizes all four ribonucleoside triphosphates as substrates, highlighting its versatility in nucleotide interactions during termination. Transcription termination factor Rho Protein, E.coli (His) is the recombinant E. coli-derived Transcription termination factor Rho protein, expressed by E. coli , with N-6*His labeled tag.
The Transcription termination factor Rho protein, crucial for the regulation of gene expression, facilitates transcription termination through a well-defined mechanism. This process involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and subsequent release of the mRNA from the DNA template. As an RNA-dependent NTPase, Rho utilizes all four ribonucleoside triphosphates as substrates, highlighting its versatility in nucleotide interactions during the termination process. Notably, the ATPase activity of Rho is susceptible to inhibition by bicyclomycin and dihydrobicyclomycin, providing potential avenues for pharmacological modulation of Rho-mediated transcription termination.
Technical Parameters
-
Species E.coli
-
Source E. coli
-
Tag N-6*His
-
Accession
P0AG30 (M1-S419)
-
Gene ID948297 [NCBI]
-
Molecular Construction
-
N-term
-
6*His
-
Rho factor (M1-S419)
Accession # P0AG30 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
RHO; CSNBAD1; Prev. RP4; Opsin-2; OPN2; Retinitis Pigmentosa 4, Autosomal Dominant; Rhodopsin; Opsin 2, Rod Pigment
-
AA Sequence
MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMKRS
-
Molecular Weight
Approximately 51 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6%trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
