Prothrombin Protein, Human (HEK293, His)
Based on 1 publication(s) in Google Scholar
Prothrombin or factor II is a key protein in a variety of physiological processes. The serine protease thrombin, derived from prothrombin, cleaves the bond behind arginine and lysine residues, converting fibrinogen to fibrin. Prothrombin Protein, Human (HEK293, His) is the recombinant human-derived Prothrombin protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Prothrombin or factor II is a key protein in a variety of physiological processes. The serine protease thrombin, derived from prothrombin, cleaves the bond behind arginine and lysine residues, converting fibrinogen to fibrin. Prothrombin Protein, Human (HEK293, His) is the recombinant human-derived Prothrombin protein, expressed by HEK293 , with C-10*His labeled tag.
The Prothrombin protein, also known as Factor II, serves as a critical player in various physiological processes. Thrombin, a serine protease derived from prothrombin, cleaves bonds following Arg and Lys residues, facilitating the conversion of fibrinogen into fibrin. Additionally, prothrombin actively participates in the activation of key factors such as V, VII, VIII, XIII, and, when in conjunction with thrombomodulin, protein C. Beyond its central role in blood homeostasis, prothrombin contributes significantly to inflammation and the intricate process of wound healing.
Publications (1)
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Journal Impact Factor
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Most Recent
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Anal Chem
Bridging Target Recognition with Direct Molecular Counting: A DNA Framework Converter-Mediated nFCM Assay Platform for Quantifying Diverse Biochemical Analytes. [Abstract]2026 Mar 17;98(10):7888-7899. PMID: 41784097
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P00734 (M1-E622)
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Gene ID2147
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Molecular Construction
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N-term
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Prothrombin (M1-E622)
Accession # P00734 -
10*His
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C-term
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Protein Length
Full Length
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Synonyms
F2; Coagulation Factor II (Thrombin); Coagulation Factor II, Thrombin; Prothrombin B-Chain; Prothrombin; F2 Protein; Prepro-Coagulation Factor II; RPRGL2; Coagulation Factor II; THPH1; Thrombin Factor II; PT
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AA Sequence
MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE
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Predicted Molecular Mass
70 kDa
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Molecular Weight
Approximately 75-100 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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