RecF/UvrF Protein, E.coli (His-SUMO)
Based on 1 Customer Validation
RecF/UvrF Protein is crucial in DNA metabolism, essential for DNA replication and inducing the SOS response. Its preference for binding to single-stranded DNA underscores its role in DNA maintenance. RecF also displays an affinity for ATP binding, emphasizing its involvement in regulating DNA-related functions. RecF/UvrF Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecF/UvrF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
RecF/UvrF Protein is crucial in DNA metabolism, essential for DNA replication and inducing the SOS response. Its preference for binding to single-stranded DNA underscores its role in DNA maintenance. RecF also displays an affinity for ATP binding, emphasizing its involvement in regulating DNA-related functions. RecF/UvrF Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecF/UvrF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
RecF/UvrF protein plays a crucial role in DNA metabolism, being essential for both DNA replication and the proper inducibility of the SOS response. Its preference for binding to single-stranded, linear DNA highlights its involvement in the intricate processes of DNA maintenance. Additionally, RecF exhibits an affinity for ATP binding, further emphasizing its role in the regulation of DNA-related functions.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-SUMO;N-6*His
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Accession
P0A7H0 (S2-D357)
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Gene ID948209 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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RecF (S2-D357)
Accession # P0A7H0 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
DNA replication and repair protein RecF; recF; uvrF; b3700; JW3677
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AA Sequence
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
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Predicted Molecular Mass
56.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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