RFP Protein, Discosoma sp. (His)
Based on 1 Customer Validation
RFP Protein, Discosoma sp. (His) is the recombinant RFP, expressed by E. coli , with His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RFP Protein, Discosoma sp. (His) is the recombinant RFP, expressed by E. coli , with His labeled tag.
Background
RFP is thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems, preventing photoinhibition caused by high light levels near the surface of coral reefs. In deeper water, its fluorescence may convert blue light into longer wavelengths more suitable for photosynthesis by the microalgal symbionts.
Technical Parameters
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Species Others
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Source E. coli
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Tag C-6*His
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Accession
Q9U6Y8 (M1-L225)
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Gene ID/
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Protein Length
Full Length
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Synonyms
DsRed; Red fluorescent protein drFP583
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AA Sequence
MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHHLFL
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Predicted Molecular Mass
32.8 kDa
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Molecular Weight
Approximately 36 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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