SCD Protein, Human (Cell-Free, His)
Based on 1 Customer Validation
SCD proteins (or stearoyl-CoA desaturases) critically catalyze the introduction of the first double bond into saturated fatty acyl-CoA substrates (such as palmitoyl-CoA and stearoyl-CoA). This process produces a mixture of 16:1 and 18:1 unsaturated fatty acids, which play a crucial role in lipid biosynthesis. SCD Protein, Human (Cell-Free, His) is the recombinant human-derived SCD protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SCD Protein, Human (Cell-Free, His) is 359 a.a., with molecular weight of 44.3 kDa.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
SCD proteins (or stearoyl-CoA desaturases) critically catalyze the introduction of the first double bond into saturated fatty acyl-CoA substrates (such as palmitoyl-CoA and stearoyl-CoA). This process produces a mixture of 16:1 and 18:1 unsaturated fatty acids, which play a crucial role in lipid biosynthesis. SCD Protein, Human (Cell-Free, His) is the recombinant human-derived SCD protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SCD Protein, Human (Cell-Free, His) is 359 a.a., with molecular weight of 44.3 kDa.
SCD (Stearoyl-CoA desaturase) is a vital enzyme that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the introduction of the first double bond into saturated fatty acyl-CoA substrates, such as palmitoyl-CoA and stearoyl-CoA. This process results in the production of a mixture of 16:1 and 18:1 unsaturated fatty acids, playing a crucial role in lipid biosynthesis. SCD is pivotal in regulating the expression of genes associated with lipogenesis and mitochondrial fatty acid oxidation, contributing to the biosynthesis of membrane phospholipids, cholesterol esters, and triglycerides. Moreover, SCD plays a key role in maintaining body energy homeostasis, highlighting its significance in fundamental cellular processes.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
O00767 (M1-G359)
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Gene ID6319
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Molecular Construction
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N-term
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10*His
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SCD (M1-G359)
Accession # O00767 -
C-term
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Protein Length
Full Length
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Synonyms
SCD; Stearoyl-CoA Desaturase Opposite Strand; Prev. SCDOS; HSCD1; FADS5; Predicted Protein Of HQ0998; SCD1; Delta-9-Desaturase; Fatty Acid Desaturase; Delta-9 Desaturase; Acyl-CoA Desaturase; MSTP008; Stearoyl-CoA Desaturase (Delta-9-Desaturase); Stearoyl
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AA Sequence
MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG
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Predicted Molecular Mass
44.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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