SCD Protein, Human (Cell-Free, His)

SCD proteins (or stearoyl-CoA desaturases) critically catalyze the introduction of the first double bond into saturated fatty acyl-CoA substrates (such as palmitoyl-CoA and stearoyl-CoA). This process produces a mixture of 16:1 and 18:1 unsaturated fatty acids, which play a crucial role in lipid biosynthesis. SCD Protein, Human (Cell-Free, His) is the recombinant human-derived SCD protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SCD Protein, Human (Cell-Free, His) is 359 a.a., with molecular weight of 44.3 kDa.

SCD (Stearoyl-CoA desaturase) is a vital enzyme that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the introduction of the first double bond into saturated fatty acyl-CoA substrates, such as palmitoyl-CoA and stearoyl-CoA. This process results in the production of a mixture of 16:1 and 18:1 unsaturated fatty acids, playing a crucial role in lipid biosynthesis. SCD is pivotal in regulating the expression of genes associated with lipogenesis and mitochondrial fatty acid oxidation, contributing to the biosynthesis of membrane phospholipids, cholesterol esters, and triglycerides. Moreover, SCD plays a key role in maintaining body energy homeostasis, highlighting its significance in fundamental cellular processes.

Human

E. coli Cell-free

N-10*His

O00767 (M1-G359)

6319

Full Length

MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.