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Siglec-E Protein, Mouse (HEK293, Fc)

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Based on 1 Customer Validation

Siglec-E Protein is a mouse orthologue of human Siglec-9 and functions as a key immunosuppressive checkpoint molecule. Siglec-E interacts with CD36 to inhibits downstream VAV signaling involved in modified LDL uptake, thereby delaying atherosclerosis. The endogenous inducible Siglec-E plays crucial anti-inflammatory and neuroprotective roles following ischemic stroke. Siglec-E Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Siglec-E protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Siglec-E Protein, Mouse (HEK293, Fc)
  • Experimental Validation Results for Siglec-E Protein, Mouse (HEK293, Fc)
  • Siglec-E Protein, Mouse (HEK293, Fc) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Siglec-E Protein is a mouse orthologue of human Siglec-9 and functions as a key immunosuppressive checkpoint molecule. Siglec-E interacts with CD36 to inhibits downstream VAV signaling involved in modified LDL uptake, thereby delaying atherosclerosis. The endogenous inducible Siglec-E plays crucial anti-inflammatory and neuroprotective roles following ischemic stroke. Siglec-E Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Siglec-E protein, expressed by HEK293 , with C-hFc labeled tag.

Background

Sialic acid binding Ig-like lectin E (Siglec-E) is a mouse orthologue of human Siglec-9 and functions as a key immunosuppressive checkpoint molecule. Siglec-E is a sialic acid binding lectin predominantly expressed on the surface of myeloid cells to transduce inhibitory signal via recruitment of SH2-domain containing protein tyrosine phosphatase SHP-1/2 upon binding to its sialoglycan ligands. Siglec-E interacts with CD36 to inhibits downstream VAV signaling involved in modified LDL uptake, thereby delaying atherosclerosis. The endogenous inducible Siglec-E plays crucial anti-inflammatory and neuroprotective roles following ischemic stroke, and thus might underlie an intrinsic mechanism of resolution of inflammation and self-repair in the brain. Siglec-E is an important negative regulator of neutrophil recruitment to the lung and β2 integrin-dependent signaling and suppresses CD11b β2-integrin-dependent signaling[1][2][3].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Mouse Siglec-E at 2 μg/mL (100 μL/well) can bind biotinylated Mouse Galectin-3. The ED50 for this effect is 0.2-0.8 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Mouse Siglec-E at 2 μg/mL (100 μL/well) can bind biotinylated Mouse Galectin-3, The ED50 for this effect is 0.2911 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q6PJ50/AAH23280 (Q20-F355)

  • Gene ID
    83382
  • Molecular Construction
    • N-term
    • Siglec-E (Q20-F355)
      Accession # Q6PJ50
    • hFc
    • C-term
  • Protein Length

    Partial

  • Synonyms

    Sialic acid binding Ig-like lectin E; Siglece; Siglecl1

  • AA Sequence

    QNPQEGFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGEPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTDVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMMMGTF

  • Molecular Weight

    Approximately 85-95 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Mouse Siglec-E at 2 μg/mL (100 μL/well) can bind biotinylated Mouse Galectin-3, The ED50 for this effect is 0.2911 μg/mL.

  • Siglec-E Protein, Mouse (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for Siglec-E Protein, Mouse (HEK293, Fc)
  • Experimental Validation Results for Siglec-E Protein, Mouse (HEK293, Fc)
  • Siglec-E Protein, Mouse (HEK293, Fc) Product Image