MAG/Siglec-4a Protein, Mouse (HEK293, His)

MAG/Siglec-4a protein mediates myelin cell-neuron interactions by binding to gangliosides, RTN4R, and RTN4RL2. It maintains normal axonal myelination and protects motor neurons from apoptosis following injury. MAG/Siglec-4a Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAG/Siglec-4a Protein, Mouse (HEK293, His) is 497 a.a., with molecular weight of 70-120 kDa.

The MAG/Siglec-4a protein is an adhesion molecule that plays a crucial role in mediating interactions between myelinating cells and neurons. It achieves this by binding to neuronal gangliosides containing sialic acid and to the glycoproteins RTN4R and RTN4RL2. While it is not necessary for initial myelination, it is involved in maintaining normal axon myelination. Additionally, it offers protection against apoptosis in motoneurons, particularly after injury, likely through its interaction with neuronal RTN4R and RTN4RL2. In adults, it is essential for preventing the degeneration of myelinated axons, and this process is likely dependent on its binding to gangliosides on the axon cell membrane. Moreover, the MAG/Siglec-4a protein acts as a negative regulator of neurite outgrowth by inhibiting axon growth longitudinally. Its inhibitory effects on neurite extension are mediated primarily through its interaction with neuronal RTN4R, RTN4RL2, and gangliosides. It binds preferentially to alpha-2,3-linked sialic acid and ganglioside Gt1b. The MAG/Siglec-4a protein can exist as a monomer or homodimer and interacts with isoform 2 of BSG through its first three N-terminal Ig-like domains.

Measured  by its ability to inhibit SH-SY5Y growth. The ED₅₀ for this effect is 3.129  μg/mL, corresponding to a specific activity is 319.59 units/mg.

Mouse

HEK293

C-6*His

P20917 (G20-P516)

17136  [NCBI]

GHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

70-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES,150 mM NaCl,1 mM EDTA, pH 7.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.