MAG/Siglec-4a Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
MAG/Siglec-4a protein mediates myelin cell-neuron interactions by binding to gangliosides, RTN4R, and RTN4RL2.It maintains normal axonal myelination and protects motor neurons from apoptosis following injury.MAG/Siglec-4a Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MAG/Siglec-4a protein mediates myelin cell-neuron interactions by binding to gangliosides, RTN4R, and RTN4RL2.It maintains normal axonal myelination and protects motor neurons from apoptosis following injury.MAG/Siglec-4a Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The MAG/Siglec-4a protein is an adhesion molecule that plays a crucial role in mediating interactions between myelinating cells and neurons. It achieves this by binding to neuronal gangliosides containing sialic acid and to the glycoproteins RTN4R and RTN4RL2. While it is not necessary for initial myelination, it is involved in maintaining normal axon myelination. Additionally, it offers protection against apoptosis in motoneurons, particularly after injury, likely through its interaction with neuronal RTN4R and RTN4RL2. In adults, it is essential for preventing the degeneration of myelinated axons, and this process is likely dependent on its binding to gangliosides on the axon cell membrane. Moreover, the MAG/Siglec-4a protein acts as a negative regulator of neurite outgrowth by inhibiting axon growth longitudinally. Its inhibitory effects on neurite extension are mediated primarily through its interaction with neuronal RTN4R, RTN4RL2, and gangliosides. It binds preferentially to alpha-2,3-linked sialic acid and ganglioside Gt1b. The MAG/Siglec-4a protein can exist as a monomer or homodimer and interacts with isoform 2 of BSG through its first three N-terminal Ig-like domains.
Verified Bioactivity
Measured by its ability to inhibit SH-SY5Y growth. The ED50 for this effect is 3.129 μg/mL, corresponding to a specific activity is 319.59 units/mg.
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-6*His
-
Accession
P20917 (G20-P516)
-
Molecular Construction
-
N-term
-
MAG (G20-P516)
Accession # P20917 -
6*His
-
C-term
-
-
Protein Length
Extracellular Domain
-
Synonyms
MAG; S-MAG; Prev. GMA; Myelin-Associated Glycoprotein; SIGLEC-4A; Sialic Acid-Binding Immunoglobulin-Like Lectin 4A; SIGLEC4A; Siglec-4a; SIGLEC4; SPG75; Myelin Associated Glycoprotein; Sialic Acid Binding Ig-Like Lectin 4A
-
AA Sequence
GHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP
-
Molecular Weight
Approximately 70-120 kDa, based on SDS-PAGE under reducing conditions.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (239 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
