SMPD1 Protein, Human (sf9, His)

SMPD1 protein converts sphingomyelin to ceramide in lysosomes and has phospholipase C activity. SMPD1 Protein, Human (sf9, His) is the recombinant human-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

SMPD1, a multifaceted enzyme, is instrumental in the dynamic conversion of sphingomyelin to ceramide, a process crucial for diverse cellular functions. This conversion occurs in the lysosomes, where SMPD1 plays a pivotal role in sphingolipid metabolism. Remarkably, SMPD1 exhibits two distinct enzymatic forms resulting from alternative trafficking of a single protein precursor, with one form targeted to the endolysosomal compartment and the other released extracellularly. Under various stress conditions, lysosomal exocytosis emerges as a major source of the secretory form, highlighting the adaptability of SMPD1 in response to cellular cues. In addition to its sphingomyelinase activity, SMPD1 contributes significantly to cholesterol export from intraendolysosomal membranes, demonstrating its involvement in lipid homeostasis. Moreover, SMPD1 showcases phospholipase C activities towards specific substrates, underscoring its versatility in lipid metabolism. Beyond lipid processing, SMPD1 exerts regulatory control over stress-induced apoptosis by modulating ceramide production, adding a layer of complexity to its multifunctional role in cellular processes.

Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is ≥1735 pmol/min/μg, as measured under the described conditions.

Human

Sf9 insect cells

C-His

P17405 (M1-P628)

6609  [NCBI]

Partial

MPRYGASLRQSCPRSGREQGQDGTAGAPGLLWMGLVLALALALALALALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPYPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLAAQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETYILNLTQANIPGAIPHWQLLYRARETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPDGSLPEAQSLWPRP

Approximately 66.3 kDa.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 25 % glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.