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SMPD1 Protein, Human (sf9, His)

Cat. No.: HY-P76710
COA Handling Instructions

SMPD1 protein converts sphingomyelin to ceramide in lysosomes and has phospholipase C activity. SMPD1 Protein, Human (sf9, His) is the recombinant human-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SMPD1 Protein, Human (sf9, His) is 628 a.a., with molecular weight of ~66.3 KDa.

For research use only. We do not sell to patients.

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Description

SMPD1 protein converts sphingomyelin to ceramide in lysosomes and has phospholipase C activity. SMPD1 Protein, Human (sf9, His) is the recombinant human-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SMPD1 Protein, Human (sf9, His) is 628 a.a., with molecular weight of ~66.3 KDa.

Background

SMPD1, a multifaceted enzyme, is instrumental in the dynamic conversion of sphingomyelin to ceramide, a process crucial for diverse cellular functions. This conversion occurs in the lysosomes, where SMPD1 plays a pivotal role in sphingolipid metabolism. Remarkably, SMPD1 exhibits two distinct enzymatic forms resulting from alternative trafficking of a single protein precursor, with one form targeted to the endolysosomal compartment and the other released extracellularly. Under various stress conditions, lysosomal exocytosis emerges as a major source of the secretory form, highlighting the adaptability of SMPD1 in response to cellular cues. In addition to its sphingomyelinase activity, SMPD1 contributes significantly to cholesterol export from intraendolysosomal membranes, demonstrating its involvement in lipid homeostasis. Moreover, SMPD1 showcases phospholipase C activities towards specific substrates, underscoring its versatility in lipid metabolism. Beyond lipid processing, SMPD1 exerts regulatory control over stress-induced apoptosis by modulating ceramide production, adding a layer of complexity to its multifunctional role in cellular processes.

Biological Activity

Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is ≥1735 pmol/min/μg, as measured under the described conditions.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P17405 (M1-P628)

Gene ID
Molecular Construction
N-term
SMPD1 (M1-P628)
Accession # P17405
His
C-term
Synonyms
Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASMase; Smpd1; Asm
Molecular Weight

Approximately 66.3 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 25 % glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

SMPD1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SMPD1 Protein, Human (sf9, His)
Cat. No.:
HY-P76710
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