Src Protein, Mouse (sf9, His-GST)

Src, a non-receptor protein tyrosine kinase, displays a unique ability to phosphorylate synaptophysin with high affinity. Src Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Src protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Src Protein, Mouse (sf9, His-GST) is 535 a.a., with molecular weight of ~80 kDa.

Src, a non-receptor protein tyrosine kinase, exhibits a distinctive capability for phosphorylating synaptophysin with high affinity.

1.The specific activity was determined to be > 50 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
2.Measured by its binding ability in a functional ELISA. Immobilized mouse SRC at 2 μg/mL (100 μL/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/mL.

Mouse

Sf9 insect cells

N-His;N-GST

P05480-2 (M1-L535)

20779  [NCBI]

Approximately 80 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.