Src Protein, Mouse (Active, sf9, His-GST)
Based on 1 Customer Validation
Src, a non-receptor protein tyrosine kinase, displays a unique ability to phosphorylate synaptophysin with high affinity.Src Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Src protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Src, a non-receptor protein tyrosine kinase, displays a unique ability to phosphorylate synaptophysin with high affinity.Src Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Src protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Src, a non-receptor protein tyrosine kinase, exhibits a distinctive capability for phosphorylating synaptophysin with high affinity.
1.The specific activity was determined to be > 50 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
2.Measured by its binding ability in a functional ELISA. Immobilized mouse SRC at 2 μg/mL (100 μL/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/mL.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
P05480-2 (M1-L535)
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Molecular Construction
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N-term
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His-GST
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Src (M1-L535)
Accession # P05480-2 -
C-term
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Protein Length
Full Length of Isoform-2
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Synonyms
SRC; Tyrosine Kinase Pp60c-Src; Prev. SRC1; P60-Src; C-Src; Protooncogene SRC, Rous Sarcoma; ASV; Tyrosine-Protein Kinase SRC-1; V-Src Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog; Pp60c-Src; Proto-Oncogene Tyrosine-Protein Kinase Src; THC6;
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AA Sequence
MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
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Predicted Molecular Mass
87.7 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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