Latent TGF beta 1/TGFB1 Protein, Rat (HEK293, His)

Latent TGF beta 1 (latent TGFB1) is the inactive form of TGF-B1. Latent TGF beta 1 associates with the extracellular matrix (ECM) via LTBP. LTBPs are components of the ECM, so that the proteolytic cleavage of LTBP can lead to the release of latent TGF-beta 1 from the matrix. Besides, the proteolytic cleavage and liberation of active TGFB1 is performed by BMP-1, by a variety of matrix metalloproteinases (MMPs)^[1][3]. Latent TGF beta 1/TGFB1 Protein, Rat (HEK293, His) is the recombinant rat-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag.

TGF-B1 is known to be secreted in the inactive, latent form. And latent TGF beta 1 (latent TGFB1) is comprised of three distinct components: mature TGFB1 which is a disulphide-bonded dimer, the N-terminal remnant of theTGFB1 precursor and a novel type of protein denoted the latent TGFB1-binding protein (LTBP)^[2].
Although latent TGF beta 1 is converted into its biologically active form by various mechanisms, but all mechanisms involve dissociation of TGFB1 from LAP-B1 in the soluble SLC (small latent complex) and/or the ECM bound LLC (large latent complex). In addition, Proteolytic cleavage is the most prominent cellular mechanism of latent TGFB1 activation. Latent TGF beta 1 associates with the extracellular matrix (ECM) via LTBP. LTBPs are components of the ECM, so that the proteolytic cleavage of LTBP can lead to the release of latent TGF-beta 1 from the matrix. Besides, the proteolytic cleavage of LLC and liberation of active TGFB1 is performed by BMP-1, by a variety of matrix metalloproteinases (MMPs)^[1][3].

Rat

HEK293

C-His

P17246 (L30-S390)

59086  [NCBI]

Full Length of Mature Protein

LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 55 kDa & 38 kDa & 16 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.