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  2. Bacterial
  3. Rf470DL

Rf470DL 

Cat. No.: HY-D1689
Handling Instructions

Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm).

For research use only. We do not sell to patients.

Rf470DL Chemical Structure

Rf470DL Chemical Structure

CAS No. : 2758364-03-1

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Description

Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm)[1].

In Vitro

Rf470DL can realize ash-free imaging of bacterial cell walls[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. RfDAA/FDAA stock solutions are prepared in DMSO at a concentration of 100 mM and stored at -20°C before use.
2.1 For long-pulse labeling of B. subtilis and E. coli, dilute the exponential phase cultures with fresh LB broth containing 1 mM Rf470DL to OD600~0.05, and incubate for 1 h.
2.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.
3.1 For short-pulse labeling of S. venezuelae, Rf470DL stock solution is added directly to exponential phase cultures to a final concentration of 0.5 mM, followed by incubating at 30 °C with shaking for 15 min.
3.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

547.07

Formula

C26H31ClN4O5S

CAS No.
SMILES

N#C/C(C(O)=O)=C\C1=CC=C(S1)C2=CC=C3C(CCCN3CCC(NCCCC[[email protected]](C(O)=O)N)=O)=C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Rf470DL
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