RNase L-IN-1 (trihydrochloride)
Based on 1 Customer Validation
RNase L-IN-1 trihydrochloride is a RNase L inhibitor with an IC50 of 15.9 μM. RNase L-IN-1 trihydrochloride binds to the ATP-binding pocket of RNase L, and inhibits ribosomal RNA cleavage. RNase L-IN-1 trihydrochloride can be used for the research of RNase L-associated cellular processes.
For research use only. We do not sell to patients.
- Purity: 98.86%
- Formula: C27H38Cl3FN6O3S
- Molecular Weight:652.05
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
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RNASE L |
RNase L-IN-1 (compound 17a) (4-250 μM; 30-30 min) trihydrochloride completely inhibits purified human full-length RNase L-mediated RNA cleavage at 250 μM, with clear dose-dependent activity at lower concentrations[1].
RNase L-IN-1 (1.56-100 μM; 400-1200 s) trihydrochloride exhibits significantly concentration-dependent binding to biotinylated purified human full-length RNase L[1].
RNase L-IN-1 (2-10 μM; 4 h) trihydrochloride efficiently inhibits poly I:C-induced rRNA cleavage in HeLa cells, with almost complete inhibition at 10 μM[1].
RNase L-IN-1 trihydrochloride binds to the ATP-binding pocket of human RNase L with improved shape complementarity and forms multiple hydrogen bonds with key residues (Lys392, Gln489, Glu404), contributing to its enhanced inhibitory potency[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 652.05
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Formula C27H38Cl3FN6O3S
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Color Brown to reddish brown
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SMILES
O=C1/C(SC(NC2=CC(F)=CC=C2)=C1C(NCCN)=O)=C\C3=C(C)C(C(NCCN(CC)CC)=O)=C(C)N3.[H]Cl.[H]Cl.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 200 mg/mL (306.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (7.67 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (7.67 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5336 mL | 7.6681 mL | 15.3362 mL | 38.3406 mL |
| 5 mM | 0.3067 mL | 1.5336 mL | 3.0672 mL | 7.6681 mL | |
| 10 mM | 0.1534 mL | 0.7668 mL | 1.5336 mL | 3.8341 mL | |
| 15 mM | 0.1022 mL | 0.5112 mL | 1.0224 mL | 2.5560 mL | |
| 20 mM | 0.0767 mL | 0.3834 mL | 0.7668 mL | 1.9170 mL | |
| 25 mM | 0.0613 mL | 0.3067 mL | 0.6134 mL | 1.5336 mL | |
| 30 mM | 0.0511 mL | 0.2556 mL | 0.5112 mL | 1.2780 mL | |
| 40 mM | 0.0383 mL | 0.1917 mL | 0.3834 mL | 0.9585 mL | |
| 50 mM | 0.0307 mL | 0.1534 mL | 0.3067 mL | 0.7668 mL | |
| 60 mM | 0.0256 mL | 0.1278 mL | 0.2556 mL | 0.6390 mL | |
| 80 mM | 0.0192 mL | 0.0959 mL | 0.1917 mL | 0.4793 mL | |
| 100 mM | 0.0153 mL | 0.0767 mL | 0.1534 mL | 0.3834 mL |
RNase L-IN-1 (trihydrochloride) Related Classifications
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