Sandoz 58-035
Based on 1 Customer Validation
Sandoz 58-035 is a selective acyl-CoA:cholesterol acyltransferase (ACAT) inhibitor. Sandoz 58-035 inhibits this enzyme in intact cells and isolated microsomal fractions. Sandoz 58-035 blocks the esterification of exogenous vesicle-derived cholesterol and the incorporation of oleic acid into cellular cholesterol esters, reducing the formation and accumulation of cholesterol esters. Sandoz 58-035 causes a slight increase in cellular free cholesterol, and at high concentrations, it also causes a slight reduction in overall cellular protein synthesis. Sandoz 58-035 can be used in studies related to cellular cholesterol regulation.
For research use only. We do not sell to patients.
- Purity: 99.34%
- CAS No.: 78934-83-5
- Formula: C30H47NOSi
- Molecular Weight:465.79
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Sandoz 58-035 (0.05-10 μg/mL; 9-24 h) potently inhibits acyl-CoA:cholesterol acyltransferase-mediated [3H] cholesterol esterification in normal human skin fibroblasts, GM 1606 fibroblasts, and GM 0863 fibroblasts, with half-maximal inhibition at 0.1 μg/mL[1].
Sandoz 58-035 (Compound 58-035) (0.12-3.3 μg/mL; 24 h) dose-dependently inhibits cholesteryl ester accumulation in Fu5AH rat hepatoma cells, achieving 93% inhibition, while slightly increasing cellular unesterified cholesterol[2].
Sandoz 58-035 (1 μg/mL; 24 h) inhibits cholesteryl ester accumulation by 73-92% in monkey aortic smooth muscle cells in hyperlipemic serum[2].
Sandoz 58-035 completely inhibits cholesteryl ester accumulation in Fu5AH rat hepatoma cells at concentrations ≥200 ng/mL[2].
Sandoz 58-035 (5 μg/mL; 0-180 min) rapidly inhibits cholesteryl [3H]oleate synthesis,[2].
Sandoz 58-035 (5 μg/mL; 2-24 h) reduces general protein synthesis in Fu5AH rat hepatoma cells[2].
Sandoz 58-035 (2.5-200 ng per incubation; 15 min pre-incubation) completely inhibits acyl-CoA:cholesterol acyltransferase activity in Fu5AH rat hepatoma cell and rat liver microsomes, while leaving at least 75% of acyl-CoA:retinol acyltransferase activity intact and increasing triglyceride synthesis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 78934-83-5
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Appearance Solid
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Molecular Weight 465.79
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Formula C30H47NOSi
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Color White to off-white
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SMILES
O=C(NC(C1=CC=CC=C1)CC2=CC=C(C)C=C2)CC[Si](C)(CCCCCCCCCC)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (214.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1469 mL | 10.7345 mL | 21.4689 mL | 53.6723 mL |
| 5 mM | 0.4294 mL | 2.1469 mL | 4.2938 mL | 10.7345 mL | |
| 10 mM | 0.2147 mL | 1.0734 mL | 2.1469 mL | 5.3672 mL | |
| 15 mM | 0.1431 mL | 0.7156 mL | 1.4313 mL | 3.5782 mL | |
| 20 mM | 0.1073 mL | 0.5367 mL | 1.0734 mL | 2.6836 mL | |
| 25 mM | 0.0859 mL | 0.4294 mL | 0.8588 mL | 2.1469 mL | |
| 30 mM | 0.0716 mL | 0.3578 mL | 0.7156 mL | 1.7891 mL | |
| 40 mM | 0.0537 mL | 0.2684 mL | 0.5367 mL | 1.3418 mL | |
| 50 mM | 0.0429 mL | 0.2147 mL | 0.4294 mL | 1.0734 mL | |
| 60 mM | 0.0358 mL | 0.1789 mL | 0.3578 mL | 0.8945 mL | |
| 80 mM | 0.0268 mL | 0.1342 mL | 0.2684 mL | 0.6709 mL | |
| 100 mM | 0.0215 mL | 0.1073 mL | 0.2147 mL | 0.5367 mL |
Sandoz 58-035 Related Classifications
HY-112487 Related Classifications
Powered by Bioz
- Sandoz 58-035
- 78934-83-5
- Acyltransferase
- GM 1606 fibroblasts
- acyl-CoA:retinol acyltransferase
- monkey aortic smooth muscle cells
- GM 0863 fibroblasts
- rat liver microsomes
- cytosomal neutral sterol ester hydrolase
- lysosomal acid sterol ester hydrolase
- acyl-CoA:cholesterol acyltransferase
- normal human skin fibroblasts
- Fu5AH rat hepatoma cells
- Inhibitor
- inhibitor
- inhibit