Annexin V-FITC/7-AAD staining kit

The discovery of hit molecule is a cornerstone of drug development. Among the diverse tools available, DNA-encoded libraries have emerged a revolutionary platform for high-throughput screening. Compared with traditional HTS, DEL features shorter screening processes, lower costs, simpler assays, and larger library capacities.

DEL Construction utilizes split-and-pool synthesis, a combinatorial chemistry approach that involves iterative splitting, reaction, and pooling. This strategy enables rapid, exponential assembly of fragments in minimal steps without the need for individual compound synthesis andassoicicated isolation or purification steps, thus greatly reducing overall costs. The technology enables simultaneous affinity screeningof massive compound collections to target proteins in a single step. By coupling chemical structures with unique DNA barcodes, each compound is tagged with a distinct DNA sequence for convenient tracking and decoding.DELs readily enable the construction and efficient screening of libraries containing millions to billions of compounds. As a result, DEL screening combines the dual advantages of high efficiency and low cost, making DEL a transformative technology in modern drug discovery.

The DEL kit consists of 50 independent libraries with a total scale of 100 billion compounds. It is constructed through stepwise combinatorial chemistry strategies involving 2-, 3-, and 4-round synthesis. By employing diverse scaffolds and flexible linking strategies, it encompasses various ring systems, linear frameworks, and heterocyclic structures. Screening can be achieved solely through affinity, independent of target-specific activity detection methods. This library is suitable for DEL screening against a wide range of targets.

Cyclic peptide library have advantages such as high affinity, high selectivity, and suitability for targeting protein–protein interactions. Through DEL synthesis technology, the library size can achieve hundreds of millions. DEL cyclic peptide library have advantages like low cost andhigh screeing efficiency, making them valuable for discovering lead compounds against challenging drug targets.

This cyclic peptide library is constructed with unnatural amino acids as building block, synthesized through DNA-compatible chemical reactions. Each cyclic peptide consist of six amino acids and constrained conformations such as side-chain cross-linking, disulfide bonds, and macrocyclization. These cyclic peptides exhibit significantly improved stability and druggability compared with linear peptides, filling the gap between small molecules and macromolecular biologics. Each cyclic peptide is uniquely conjugated to a DNA barcode sequence for molecular identification and sequencing decoding.

MCE’s cyclic peptide library has8 independent sub-libraries, with a total molecular diversity of 1.2 billion. It is constructed via multi-round combinatorial assembly of building blocks and diverse cyclization strategies, facilitating the discovery of cyclic peptide leads for undruggable targets.

MCE Annexin V-FITC/7-AAD Apoptosis Detection Kit can be utilized to detect the occurrence of early apoptosis.

MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).

MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-),, early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).

Annexin V-mCherry Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

MCE Annexin V-PE Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

Annexin V-mCherry/SYTOX Green Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells show red fluorescence, necrosis cells show red and green fluorescence.

MCE VF 647A-Annexin V/PI Apoptosis Detection Kit can be utilized to detect the level of cell apoptosis.

MCE Hematoxylin-Eosin Staining Kit can be used for cell staining, the combination of hematoxylin and eosin results in a staining pattern where the cytoplasm appears red or pink and the nucleus takes on a blue or blue-violet.

MCE Hematoxylin-Eosin Staining Kit (Including Differentiation and Bluing Solutions) contains staining solutions, differentiation solution, and bluing solution. Through optimized formulations, it helps to adjust the staining effect, ensuring proper staining intensity and further improving experimental accuracy.

MCE Masson Staining Kit can simultaneously stain various tissue components, such as cell nuclei, collagen fibers, and muscle fibers. It features low toxicity, environmental friendliness, simple operation, and stable performance. The staining results show clear coloration and high contrast. The stained sections can be stored for long periods with minimal fading, facilitating long-term preservation and image analysis. This kit is widely used in studies of connective tissue, muscle tissue, and collagen fibers, and is suitable for histological observation and related pathological analyses.

MCE Gram Stain Kit can be used to differentiate between Gram-negative and Gram-positive bacteria.

MCE Modified Masson Staining Kit employs Celestine Blue hematoxylin for light nuclear staining, offering shorter differentiation time compared to conventional methods. It features low toxicity, environmental friendliness, simple operation, and stable performance. The staining results show clear coloration and high contrast. The stained sections can be stored for long periods with minimal fading, facilitating long-term preservation and image analysis. This kit is widely used in studies of connective tissue, muscle tissue, and collagen fibers, and is suitable for histological observation and related pathological analyses.

MCE Cell Senescence β-Galactosidase Staining Kit can be used to evaluate the senescence of cells or tissues.

MCE Cell Apoptosis Analysis Kit (Hoechst staining) provides a rapid and convenient method to detect cell apoptosis.

MCE Oil Red O Staining Kit for Cultured Cells consists of Oil Red O staining solution and hematoxylin. It is used to demonstrate fat degeneration and abnormal lipid deposition in cultured cells, particularly when multiple neutral fat droplets are present within the cells. This kit helps in identifying lipid changes and their nature in cultured cells. It is important to note that samples should not be fixed with ethanol-containing fixatives, as ethanol may interfere with lipid staining. The positive staining result for fat typically appears orange-yellow to red, with the exact color depending on the lipid concentration.

MCE Oil Red O Staining Kit for Cell Smears effectively stains lipid droplets of various sizes, including smaller lipid droplets, and preferentially adsorbs dye from the solvent. It is suitable for staining oil red O in cell smears, bone marrow smears, fluid smears, blood smears, and other samples. When using the kit, specimens should not be fixed with fixatives containing ethanol. If fixation is required, 10% formalin can be used. The positive staining result for fat typically appears orange-yellow to red, with the exact color varying depending on the lipid concentration.

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.

MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show green fluorescence.

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.

MCE Sirius Red Staining Kit consists of hematoxylin staining solution and Sirius Red staining solution and is mainly used to observe abnormal collagen deposition or fibrosis in various pathological tissues. Under a conventional light microscope, collagen fibers in tissues such as the heart and blood vessels appear red. Under polarized light microscopy, this method is useful for the classification and evaluation of different types of fibrotic lesions.

MCE AB—PAS Staining Kit for Glycogen and Mucopolysaccharides combines Alcian Blue staining and PAS staining, allowing the simultaneous detection and differentiation of different types of mucins within the same tissue section. In this method, acidic mucins are typically stained blue, neutral mucins appear red or magenta, and mucins containing both acidic and neutral components may appear purple or bluish-purple. This technique is widely used in histological and pathological studies for the identification and distribution analysis of mucin types within tissues.

MCE Modified Safranin O–Fast Green Cartilage Staining Kit through an optimized staining system, cartilage matrix and bone tissue in histological sections can be clearly distinguished. This method provides high staining contrast, good sensitivity, simple operation, and good reproducibility, and is widely used for cartilage morphology observation, studies of cartilage injury and degeneration, osteochondral tissue development research, and related histological analyses.

MCE Weigert Elastin Staining Kit combines Weigert Resorcinol Fuchsin Staining Solution with Van Gieson (VG) Staining Solution. Weigert Resorcinol Fuchsin Solution is mainly used for staining elastic fibers, Van Gieson (VG) Solution is used for collagen fiber staining. The principle of VG staining is based on differences in the size of anionic dye molecules and the permeability of tissues. Picric acid (PA) has the smallest molecular weight and preferentially enters dense structures. Ponceau Red or acid fuchsin has a relatively larger molecular weight and primarily binds to collagen fibers, while light green has the largest molecular weight and stains other tissue components. After VG staining, collagen fibers appear red, while muscle fibers and cytoplasm appear yellow, enabling a clear distinction between tissue components.

MCE Modified Van Gieson Staining Kit employs Celestine Blue and Mayer’s hematoxylin for nuclear staining, providing clearer and more stable nuclear visualization while facilitating longer preservation of stained sections. Ponceau S is used for collagen fiber staining, offering stable coloration and strong resistance to fading. This method enables effective differentiation between collagen fibers and muscle fibers, and can assist in distinguishing collagen fiber–derived tumors from myogenic tumors to a certain extent. It is also suitable for observing tissue or organ injury, repair processes, and the degree of fibrosis.

MCE DAB Peroxidase Substrate Kit (Purple-Blue Color) can be used for staining and color development detection in experiments such as immunohistochemistry of cells or tissues, in situ hybridization, Western blotting, and for visualizing endogenous HRP in cells or tissues.

MCE Periodic Acid-Schiff (PAS) Staining Kit is developed based on the classical Periodic Acid–Schiff (PAS) reaction principle and is suitable for the detection of glycogen and polysaccharide-containing substances in paraffin-embedded or frozen tissue sections.

MCE Live & Dead Bacterial Viability and Counting Kit is based on dual-fluorescence staining technology, which can effectively differentiate live bacteria from dead bacteria and enable quantitative detection using flow cytometry. It can also be used for quantitative analysis of various bacterial mixtures.

MCE Viability/Cytotoxicity Assay Kit for Live & Dead Cells (Calcein/PI) enables dual fluorescent staining of both live and dead cells, making it suitable for assessing cell viability and cytotoxicity. The 500 T is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Methenamine Silver Stain Kit For Basement Membrane (PASM) is developed based on the classical staining principle. Through an optimized staining system and standardized reagent combination, it enables clear visualization of basement membranes and related reticular structures in tissue sections. This method is particularly widely used in renal pathology research, where it is commonly applied to examine morphological alterations of the glomerular capillary basement membrane, such as thickening, rupture, folding, double-contour (tram-track) appearance, or abnormal proliferation caused by inflammatory injury. In addition, this method can also be applied to the histological investigation and morphological observation of glomerular diseases, diabetic nephropathy, and other basement membrane–associated pathological changes.

MCE ATPase Staining Kit for Muscle Fibers (Calcium Activation Method) is based on the hydrolysis of ATP by ATPase, which generates ADP and inorganic phosphate. Phosphate combines with calcium ions to form colorless calcium phosphate precipitates. Calcium phosphate is then treated with cobalt chloride to form cobalt phosphate, which, after treatment with sulfide solution, results in the formation of brown-black cobalt sulfide precipitates at the enzyme activity sites.

MCE Red Nucleic Acid Gel Stain (10,000×) is a nucleic acid stain that can be used as a safer alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acids in agarose gels or polyacrylamide gels.

MCE SYBR Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels.

MCE Organoid Vitality Assay Kit is a fluorescence-based assay kit specifically designed for measuring cell viability in organoids.

MCE Ponceau S staining solution (ready-to-use, 1×) is a ready-to-use membrane staining solution that can be used for protein detection on PVDF membranes, nitrocellulose membranes, and cellulose acetate membranes.