Sodium α-ketoisocaproate
Based on 1 publication(s) in Google Scholar
Sodium α-ketoisocaproate (Sodium 4-methyl-2-oxopentanoate) is an insulin secretagogue. Sodium α-ketoisocaproate undergoes transamination catalyzed by BCAT and BCATm to produce α-ketoglutarate and leucine; it also binds to the SUR1 site to inhibit KATP channel activity, but does not directly affect the Kir6.2 subunit. Sodium α-ketoisocaproate regulates Ca2+ influx. Sodium α-ketoisocaproate exerts insulinotropic activity in pancreatic islets and intact mouse pancreata with sufficient BCATm expression. Sodium α-ketoisocaproate can be used in research related to type 2 diabetes.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.0%
- CAS 番号: 4502-00-5
- 分子式: C6H9NaO3
- 分子量:152.12
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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MedChemExpress（MCE）の使用を引用している文献 Sodium α-ketoisocaproate
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生物活性
α-ketoisocaproate (15 mM; 45 min) sodium potently stimulates insulin secretion from isolated islets, among which BTBR and (B6 × BTBR) F1 islets exhibit a hyper-responsive phenotype compared with B6 islets[1].
α-ketoisocaproate (15 mM) sodium has no inhibitory effect on the activity of KIR6.2ΔC26 channels in inside-out patches of transfected COS-7 cells[2].
Sodium α-ketoisocaproate binds to native and recombinant SUR1, as well as recombinant SUR2B, with a Kd value range of 46.9~57.2 mM, indicating its low affinity for sulfonylurea receptor sites[2].
Sodium α-ketoisocaproate inhibits the KATP channel current in inside-out membrane patches of mouse pancreatic β cells, with an IC50 of 18.9 mM, and exerts only extremely weak inhibition at the estimated physiological cytoplasmic concentration[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 4502-00-5
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性状 Solid
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分子量 152.12
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分子式 C6H9NaO3
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Color White to yellow
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SMILES
[Na].O=C(O)C(=O)CC(C)C
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別名
Sodium 4-methyl-2-oxopentanoate; 2-Ketoisocaproic acid sodium salt
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Branched-Chain α Keto-Acid Dehydrogenase Kinase-Mediated AKT Phosphorylation Promotes RCC Tumorigenesis and Drug Resistance. [Abstract]2025 Aug 11:e11081. PMID: 40789057
溶剤 & 溶解度
H2O : 50 mg/mL (328.69 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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データシート (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
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- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Rabaglia ME, et al. Alpha-Ketoisocaproate-induced hypersecretion of insulin by islets from diabetes-susceptible mice. Am J Physiol Endocrinol Metab. 2005;289(2):E218-E224. [Content Brief]
[2]. Heissig H, et al. Mechanism of the insulin-releasing action of alpha-ketoisocaproate and related alpha-keto acid anions. Mol Pharmacol. 2005 Oct;68(4):1097-105. [Content Brief]
[3]. Zhou Y, et al. Transamination is required for {alpha}-ketoisocaproate but not leucine to stimulate insulin secretion. J Biol Chem. 2010 Oct 29;285(44):33718-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.5738 mL | 32.8688 mL | 65.7376 mL | 164.3439 mL |
| 5 mM | 1.3148 mL | 6.5738 mL | 13.1475 mL | 32.8688 mL | |
| 10 mM | 0.6574 mL | 3.2869 mL | 6.5738 mL | 16.4344 mL | |
| 15 mM | 0.4383 mL | 2.1913 mL | 4.3825 mL | 10.9563 mL | |
| 20 mM | 0.3287 mL | 1.6434 mL | 3.2869 mL | 8.2172 mL | |
| 25 mM | 0.2630 mL | 1.3148 mL | 2.6295 mL | 6.5738 mL | |
| 30 mM | 0.2191 mL | 1.0956 mL | 2.1913 mL | 5.4781 mL | |
| 40 mM | 0.1643 mL | 0.8217 mL | 1.6434 mL | 4.1086 mL | |
| 50 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2869 mL | |
| 60 mM | 0.1096 mL | 0.5478 mL | 1.0956 mL | 2.7391 mL | |
| 80 mM | 0.0822 mL | 0.4109 mL | 0.8217 mL | 2.0543 mL | |
| 100 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6434 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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