Sulfo-ara-F-NMN (Synonyms: CZ-48)

Name: Sulfo-ara-F-NMN
Brand: MedChemExpress
SKU: HY-129522
Rating: 5.0 (6 reviews)

Cat. No.: HY-129522 Purity: 99.18%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Sulfo-ara-F-NMN (CZ-48) is a mimetic of nicotinamide mononucleotide (NMN). Sulfo-ara-F-NMN acts selectively, activating SARM1 but inhibiting CD38 (IC₅₀ around 10 μM). Sulfo-ara-F-NMN induces intracellular cyclic ADP-ribose (cADPR) production.

For research use only. We do not sell to patients.

CAS No. : 1374663-29-2

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 6 publication(s) in Google Scholar

6 Publications Citing Use of MCE Sulfo-ara-F-NMN

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

: Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: Cell. 2025 Dec 11;188(25):7137-7154.e21. [Abstract]; Response curves of intracellular cADPR production and NAD⁺ consumption in the HEK293T cells treated with Sulfo-ara-F-NMN (CZ-48) (1-4 mM). HEK293T cells stably expressing WT hSARM1 were treated with different doses of CZ-48 for 24 h. cADPR and NAD⁺ levels in the resultant cells were assessed by MS.

: Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: Cell. 2025 Dec 11;188(25):7137-7154.e21. [Abstract]; Representative IF images of the noted mouse neural cells treated with dsDNA transfection or Sulfo-ara-F-NMN (CZ-48). The noted neural cells were treated with 2 μg/mL dsDNA transfection per 16 h or 1 mM CZ-48. After 60 h, the resultant cells were stained with anti-SARM1 antibody. CZ-48 promoted both WT and 3KE neural cell death, while dsDNA transfection promoted only WT cell death. BF, bright field; red, mSARM1; blue, DAPI. Scale bar, 10 μm.

: Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Aug 22. [Abstract]; Sulfo-ara-F-NMN (CZ48) (1–1000 μM; 24 h) acted as an NMN-mimicking SARM1 activator and induced SARM1-dependent cell death in HEK-293T cells overexpressing SARM1dN.

: Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 8;27(6):109940.; MTT conversion to formazan at day 2 of PMA-differentiated THP-1 cells. Cells were treated with PMA for 24 h followed by being cultured in PMA-free, containing indicated stimulants media for 24 h. Unstimulated cells are used as normalization, set as 100%. DMSO is used as a control for Sulfo-ara-F-NMN (CZ-48) (100 μM; 24 h) and it’s also treated in cells shown as LPS. Sulfo-ara-F-NMN (CZ-48) treatment significantly reduced MTT conversion in SARM1-WT but not SARM1-E642A nor EV cells, both with or without LPS.

: Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 8;27(6):109940.; Left panel, PMA differentiated THP-1 cells were stimulated with LPS and/or Sulfo-ara-F-NMN (CZ-48) (100 μM) for 6 h. Immunoblots of pro-IL-1β and SARM1 (to show no effect by NADase in other proteins) from cell lysates of PMA-differentiated THP-1 stimulated with LPS and/or CZ-48. β-actin was used as a loading control. Right panel, quantification of pro-IL-1β.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Sterile alpha and Toll/interleukin-1 receptor motif-containing 1 (SARM1) is an adaptor protein in the Toll-like receptor pathway. Sulfo-ara-F-NMN activates SARM1 to produce cyclic ADP-ribose and induces non-apoptotic cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

352.28

Formula

C₁₁H₁₄FN₂O₆PS

CAS No.

1374663-29-2

Appearance

Solid

Color

White to light yellow

SMILES

O=C(N)C1=CC=C[N+]([C@@H]2O[C@H](COP([O-])(O)=S)[C@@H](O)[C@@H]2F)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (283.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (283.87 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8387 mL	14.1933 mL	28.3865 mL
5 mM	0.5677 mL	2.8387 mL	5.6773 mL
10 mM	0.2839 mL	1.4193 mL	2.8387 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.36%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (252 KB) HNMR (318 KB) LCMS (122 KB)

Handling Instructions (2659 KB)

References

[1]. Zhao ZY, et al. A Cell-Permeant Mimetic of NMN Activates SARM1 to Produce Cyclic ADP-Ribose and Induce Non-apoptotic Cell Death. iScience. 2019 May 31;15:452-466. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.8387 mL	14.1933 mL	28.3865 mL	70.9663 mL
	5 mM	0.5677 mL	2.8387 mL	5.6773 mL	14.1933 mL
	10 mM	0.2839 mL	1.4193 mL	2.8387 mL	7.0966 mL
	15 mM	0.1892 mL	0.9462 mL	1.8924 mL	4.7311 mL
	20 mM	0.1419 mL	0.7097 mL	1.4193 mL	3.5483 mL
	25 mM	0.1135 mL	0.5677 mL	1.1355 mL	2.8387 mL
	30 mM	0.0946 mL	0.4731 mL	0.9462 mL	2.3655 mL
	40 mM	0.0710 mL	0.3548 mL	0.7097 mL	1.7742 mL
	50 mM	0.0568 mL	0.2839 mL	0.5677 mL	1.4193 mL
	60 mM	0.0473 mL	0.2366 mL	0.4731 mL	1.1828 mL
	80 mM	0.0355 mL	0.1774 mL	0.3548 mL	0.8871 mL
	100 mM	0.0284 mL	0.1419 mL	0.2839 mL	0.7097 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Sulfo-ara-F-NMN Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfo-ara-F-NMN1374663-29-2CZ-48CZ48CZ 48Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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Sulfo-ara-F-NMN (Synonyms: CZ-48)

Customer Review

Sulfo-ara-F-NMN Related Antibodies

6 Publications Citing Use of MCE Sulfo-ara-F-NMN

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•Related Screening Libraries:

•Related Small Molecules:

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View All Toll-like Receptor (TLR) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sulfo-ara-F-NMN Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Sulfo-ara-F-NMN Related Classifications

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Customer Review

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