Sulfo-ara-F-NMN
Based on 7 publication(s) in Google Scholar
Sulfo-ara-F-NMN (CZ-48) is a mimetic of nicotinamide mononucleotide (NMN). Sulfo-ara-F-NMN acts selectively, activating SARM1 but inhibiting CD38 (IC50 around 10 μM). Sulfo-ara-F-NMN induces intracellular cyclic ADP-ribose (cADPR) production.
For research use only. We do not sell to patients.
- Purity: 99.18%
- CAS No.: 1374663-29-2
- Formula: C11H14FN2O6PS
- Molecular Weight:352.28
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Storage:
-20°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Sulfo-ara-F-NMN
More- Cell. 2025 Dec 11;188(25):7137-7154.e21. [Abstract]
- Nat Chem Biol. 2025 Aug 22. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Feb 25;122(8):e2424906122. [Abstract]
- J Med Chem. 2026 Apr 23;69(8):9521-9536. [Abstract]
- iScience. 2024 May 8;27(6):109940. [Abstract]
- J Neurosci. 2024 Jun 12;44(24):e2424232024. [Abstract]
- bioRxiv. 2024 November 25.
Customer Validation & Images
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Bio/Physico-chemical Assay
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IF
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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WB
Biological Activity
Sterile alpha and Toll/interleukin-1 receptor motif-containing 1 (SARM1) is an adaptor protein in the Toll-like receptor pathway. Sulfo-ara-F-NMN activates SARM1 to produce cyclic ADP-ribose and induces non-apoptotic cell death[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1374663-29-2
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Appearance Solid
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Molecular Weight 352.28
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Formula C11H14FN2O6PS
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Color White to light yellow
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SMILES
O=C(N)C1=CC=C[N+]([C@@H]2O[C@H](COP([O-])(O)=S)[C@@H](O)[C@@H]2F)=C1
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Synonyms
CZ-48
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (7)
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Journal Impact Factor
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Most Recent
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Cell
2025 Dec 11;188(25):7137-7154.e21. PMID: 41138726
Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: Cell. 2025 Dec 11;188(25):7137-7154.e21. [Abstract]
Response curves of intracellular cADPR production and NAD+ consumption in the HEK293T cells treated with Sulfo-ara-F-NMN (CZ-48) (1-4 mM). HEK293T cells stably expressing WT hSARM1 were treated with different doses of CZ-48 for 24 h. cADPR and NAD+ levels in the resultant cells were assessed by MS.
Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: Cell. 2025 Dec 11;188(25):7137-7154.e21. [Abstract]
Representative IF images of the noted mouse neural cells treated with dsDNA transfection or Sulfo-ara-F-NMN (CZ-48). The noted neural cells were treated with 2 μg/mL dsDNA transfection per 16 h or 1 mM CZ-48. After 60 h, the resultant cells were stained with anti-SARM1 antibody. CZ-48 promoted both WT and 3KE neural cell death, while dsDNA transfection promoted only WT cell death. BF, bright field; red, mSARM1; blue, DAPI. Scale bar, 10 μm.
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Nat Chem Biol
2025 Aug 22. PMID: 40846996
Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Aug 22. [Abstract]
Sulfo-ara-F-NMN (CZ48) (1–1000 μM; 24 h) acted as an NMN-mimicking SARM1 activator and induced SARM1-dependent cell death in HEK-293T cells overexpressing SARM1dN.
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Proc Natl Acad Sci U S A
Stepwise activation of SARM1 for cell death and axon degeneration revealed by a biosynthetic NMN mimic. [Abstract]2025 Feb 25;122(8):e2424906122. PMID: 39964720 -
J Med Chem
Structure-Based Discovery of Imidazo[4,5- c]pyridine SARM1 Modulators Showing Paradoxical Activation. [Abstract]2026 Apr 23;69(8):9521-9536. PMID: 41948869 -
iScience
SARM1 regulates pro-inflammatory cytokine expression in human monocytes by NADase-dependent and -independent mechanisms. [Abstract]2024 May 8;27(6):109940. PMID: 38832024
Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 8;27(6):109940. [Abstract]
MTT conversion to formazan at day 2 of PMA-differentiated THP-1 cells. Cells were treated with PMA for 24 h followed by being cultured in PMA-free, containing indicated stimulants media for 24 h. Unstimulated cells are used as normalization, set as 100%. DMSO is used as a control for Sulfo-ara-F-NMN (CZ-48) (100 μM; 24 h) and it’s also treated in cells shown as LPS. Sulfo-ara-F-NMN (CZ-48) treatment significantly reduced MTT conversion in SARM1-WT but not SARM1-E642A nor EV cells, both with or without LPS.
Sulfo-ara-F-NMN purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 8;27(6):109940. [Abstract]
Left panel, PMA differentiated THP-1 cells were stimulated with LPS and/or Sulfo-ara-F-NMN (CZ-48) (100 μM) for 6 h. Immunoblots of pro-IL-1β and SARM1 (to show no effect by NADase in other proteins) from cell lysates of PMA-differentiated THP-1 stimulated with LPS and/or CZ-48. β-actin was used as a loading control. Right panel, quantification of pro-IL-1β.
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J Neurosci
Context-specific stress causes compartmentalized SARM1 activation and local degeneration in cortical neurons. [Abstract]2024 Jun 12;44(24):e2424232024. PMID: 38692735 -
Solvent & Solubility
DMSO : 100 mg/mL (283.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (283.87 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.10 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.10 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 2.8387 mL | 14.1933 mL | 28.3865 mL | 70.9663 mL |
| 5 mM | 0.5677 mL | 2.8387 mL | 5.6773 mL | 14.1933 mL | |
| 10 mM | 0.2839 mL | 1.4193 mL | 2.8387 mL | 7.0966 mL | |
| 15 mM | 0.1892 mL | 0.9462 mL | 1.8924 mL | 4.7311 mL | |
| 20 mM | 0.1419 mL | 0.7097 mL | 1.4193 mL | 3.5483 mL | |
| 25 mM | 0.1135 mL | 0.5677 mL | 1.1355 mL | 2.8387 mL | |
| 30 mM | 0.0946 mL | 0.4731 mL | 0.9462 mL | 2.3655 mL | |
| 40 mM | 0.0710 mL | 0.3548 mL | 0.7097 mL | 1.7742 mL | |
| 50 mM | 0.0568 mL | 0.2839 mL | 0.5677 mL | 1.4193 mL | |
| 60 mM | 0.0473 mL | 0.2366 mL | 0.4731 mL | 1.1828 mL | |
| 80 mM | 0.0355 mL | 0.1774 mL | 0.3548 mL | 0.8871 mL | |
| 100 mM | 0.0284 mL | 0.1419 mL | 0.2839 mL | 0.7097 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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