Tetradecylphosphocholine
Tetradecylphosphocholine is a zwitterionic surfactant that can be used as a detergent for membrane protein solubilization.
For research use only. We do not sell to patients.
- CAS No.: 77733-28-9
- Formula: C19H42NO4P
- Molecular Weight:379.51
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-W040217
MEGA-10Solubilizer -
HY-W127785
N,N-Dimethyldodecylamine N-oxideBiochemical Assay Reagent -
HY-W040218
MEGA-8Surfactant -
HY-W127785R
N,N-Dimethyldodecylamine N-oxide (Standard)Biochemical Assay Reagent -
HY-W040216
MEGA-9Non-ionic Surfactant -
HY-158724
CalxglukDetergent -
HY-158883
ODGDetergent -
HY-158884
CALXCHOL sodiumDetergent -
HY-158882
FLAC6Fluorinated Detergent -
HY-131694
N-DodecyllactobionamideSugar Surfactant -
HY-158881
DDGDetergent -
HY-168379
DLACDetergent
Biological Activity
Description
Chemical Information
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CAS No. 77733-28-9
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Appearance Solid
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Molecular Weight 379.51
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Formula C19H42NO4P
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Color White to off-white
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SMILES
CCCCCCCCCCCCCCOP(OCC[N+](C)(C)C)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 15 mg/mL (39.52 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Membrane Protein Extraction Using Detergents and Chaotropes
Membrane protein extraction with detergents and chaotropes solubilizes lipid-bilayer-associated proteins by disrupting protein-lipid and protein-protein interactions while maintaining proteins in a soluble state for downstream electrophoresis, purification, or mass spectrometry. Chaotropes such as urea and thiourea improve solubilization of difficult proteins, while nonionic and zwitterionic detergents such as CHAPS, ASB-14, SB 3-10, MEGA-10, dodecyl maltoside, and Triton X-100 differ in extraction efficiency depending on sample type and membrane protein properties.
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Inclusion-body expression, solubilization, refolding and purification
Inclusion-body recovery uses insoluble recombinant protein aggregates from E. coli as a starting material; the workflow is cell disruption, inclusion-body isolation/washing, denaturant or mild solubilization, refolding into soluble protein, and final chromatographic purification. The readouts are soluble protein recovery, purity by SDS-PAGE/chromatography, structural recovery by methods such as circular dichroism when used, and biological activity when an assay is available.
Purity & Documentation
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Data Sheet (262 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6350 mL | 13.1749 mL | 26.3498 mL | 65.8744 mL |
| 5 mM | 0.5270 mL | 2.6350 mL | 5.2700 mL | 13.1749 mL | |
| 10 mM | 0.2635 mL | 1.3175 mL | 2.6350 mL | 6.5874 mL | |
| 15 mM | 0.1757 mL | 0.8783 mL | 1.7567 mL | 4.3916 mL | |
| 20 mM | 0.1317 mL | 0.6587 mL | 1.3175 mL | 3.2937 mL | |
| 25 mM | 0.1054 mL | 0.5270 mL | 1.0540 mL | 2.6350 mL | |
| 30 mM | 0.0878 mL | 0.4392 mL | 0.8783 mL | 2.1958 mL |
Tetradecylphosphocholine Related Classifications
HY-W019895 Related Classifications
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