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Cat. No.: HY-146021

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Topoisomerase IIα-IN-2

Topoisomerase IIα-IN-2 (compound 5) is a potent DNA-binding ligands and topoisomerase IIα inhibitor. Topoisomerase IIα-IN-2 exhibits high antiproliferative activity against human cancer cell lines. Topoisomerase IIα-IN-2 significantly induces DNA damage and arrests cancer cells at G2/M phase.

For research use only. We do not sell to patients.

CAS No.: 2407521-90-6
Formula: C₂₂H₂₀N₄O₅
Molecular Weight:420.42

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (267 KB)

Topoisomerase IIα-IN-2 Chemical Structure

CAS No.: 2407521-90-6

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

topoisomerase II alpha

topoisomerase II beta

All Topoisomerase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
ACHN	IC₅₀	0.005 μM Compound: 5	Antiproliferative activity against human ACHN cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human ACHN cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
CCRF-CEM	IC₅₀	0.003 μM Compound: 5	Antiproliferative activity against human CCRF-CEM cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human CCRF-CEM cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
CCRF-SB	IC₅₀	0.005 μM Compound: 5	Antiproliferative activity against human CCRF-SB cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human CCRF-SB cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
G-361	IC₅₀	0.006 μM Compound: 5	Antiproliferative activity against human G-361 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human G-361 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
HeLa	IC₅₀	0.005 μM Compound: 5	Antiproliferative activity against human HeLa cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human HeLa cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
HT-29	IC₅₀	0.004 μM Compound: 5	Antiproliferative activity against human HT-29 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
MCF7	IC₅₀	0.003 μM Compound: 5	Antiproliferative activity against human MCF7 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
MT4	IC₅₀	0.006 μM Compound: 5	Antiproliferative activity against human MT-4 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human MT-4 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]
SK-MEL-28	IC₅₀	0.002 μM Compound: 5	Antiproliferative activity against human SKMEL-28 cells assessed as cell viability measured after 4 days by MTT assay Antiproliferative activity against human SKMEL-28 cells assessed as cell viability measured after 4 days by MTT assay	[PMID: 31869654]

In Vitro

Topoisomerase IIα-IN-2 (compound 5) shows antiproliferative activity in cancer cell lines (CCRF-CEM, CCRF-SB, MT-4, HT-29, MCF-7, HeLa, ACHN, SKMEL-28, and G-361), with IC₅₀ values of 0.003, 0.005, 0.006, 0.004, 0.003, 0.005, 0.005, 0.002, and 0.006 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2407521-90-6
Molecular Weight 420.42
Formula C₂₂H₂₀N₄O₅
SMILES

O=C1C2=NC=CC=C2C(C(OC3=C4)=C1)=NC3=CC=C4C(NCCCC[C@H](N)C(O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Pedatella S, et al. Antitumor agents 7. Synthesis, antiproliferative activity and molecular modeling of new l-lysine-conjugated pyridophenoxazinones as potent DNA-binding ligands and topoisomerase IIα inhibitors. Eur J Med Chem. 2020 Feb 1;187:111960. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Topoisomerase IIα-IN-2

All Topoisomerase Isoforms