BG-DBCO
Based on 1 Customer Validation
BG-DBCO (SNAP-DBCO) is a key bifunctional reagent that acts as a "bridge" in the ClickRNA-PROTAC system. BG-DBCO possesses a SNAPTag covalent anchoring handle and a copper-free click chemistry handle: one end covalently locks SNAPTag fusion proteins via BG, while the other end captures azide-modified target protein ligands through copper-free click chemistry (SPAAC), thereby assembling the "exogenous E3 fusion protein" and "POI ligand" into a complete PROTAC ternary complex in living cells.
For research use only. We do not sell to patients.
- Purity : 98.06%
- CAS No.: 1820756-74-8
- Formula: C32H27N7O3
- Molecular Weight:557.60
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
BG-DBCO exhibits biocompatibility when coexisting with SIAH1-SN mRNA and maintains continuous cell survival[1].
BG-DBCO enables tumor-specific assembly of functional PROTAC complexes in trigger mRNA-expressing tumor cells, mediating targeted ubiquitination and degradation of the intended target protein[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1820756-74-8
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Appearance Solid
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Molecular Weight 557.60
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Formula C32H27N7O3
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Color White to off-white
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SMILES
NC1=NC2=C(C(OCC3=CC=C(C=C3)CNC(CCC(N4CC5=C(C#CC6=CC=CC=C46)C=CC=C5)=O)=O)=N1)N=CN2
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Synonyms
SNAP-DBCO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 11.67 mg/mL (20.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7934 mL | 8.9670 mL | 17.9340 mL | 44.8350 mL |
| 5 mM | 0.3587 mL | 1.7934 mL | 3.5868 mL | 8.9670 mL | |
| 10 mM | 0.1793 mL | 0.8967 mL | 1.7934 mL | 4.4835 mL | |
| 15 mM | 0.1196 mL | 0.5978 mL | 1.1956 mL | 2.9890 mL | |
| 20 mM | 0.0897 mL | 0.4484 mL | 0.8967 mL | 2.2418 mL |
BG-DBCO Related Classifications
HY-W1124386 Related Classifications
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