2,5-Dioxopyrrolidin-1-yl hept-6-ynoate
Based on 1 Customer Validation
2,5-Dioxopyrrolidin-1-yl hept-6-ynoate is an amine reactive four carbon reagent that can be used for derivatizing peptides, antibodies, amine coated surfaces etc. The alkyne group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 917222-23-2
- Formula: C11H13NO4
- Molecular Weight:223.23
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 917222-23-2
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Appearance Solid
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Molecular Weight 223.23
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Formula C11H13NO4
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Color White to off-white
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SMILES
C#CCCCCC(ON1C(CCC1=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (447.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (242 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.4797 mL | 22.3984 mL | 44.7968 mL | 111.9921 mL |
| 5 mM | 0.8959 mL | 4.4797 mL | 8.9594 mL | 22.3984 mL | |
| 10 mM | 0.4480 mL | 2.2398 mL | 4.4797 mL | 11.1992 mL | |
| 15 mM | 0.2986 mL | 1.4932 mL | 2.9865 mL | 7.4661 mL | |
| 20 mM | 0.2240 mL | 1.1199 mL | 2.2398 mL | 5.5996 mL | |
| 25 mM | 0.1792 mL | 0.8959 mL | 1.7919 mL | 4.4797 mL | |
| 30 mM | 0.1493 mL | 0.7466 mL | 1.4932 mL | 3.7331 mL | |
| 40 mM | 0.1120 mL | 0.5600 mL | 1.1199 mL | 2.7998 mL | |
| 50 mM | 0.0896 mL | 0.4480 mL | 0.8959 mL | 2.2398 mL | |
| 60 mM | 0.0747 mL | 0.3733 mL | 0.7466 mL | 1.8665 mL | |
| 80 mM | 0.0560 mL | 0.2800 mL | 0.5600 mL | 1.3999 mL | |
| 100 mM | 0.0448 mL | 0.2240 mL | 0.4480 mL | 1.1199 mL |
2,5-Dioxopyrrolidin-1-yl hept-6-ynoate Related Classifications
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