2. Cell Cycle/DNA Damage
  3. Nucleoside Antimetabolite/Analog
  4. 2-Chloro-2′-β-C-methyladenosine

2-Chloro-2′-β-C-methyladenosine is an adenosine analogue. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. The popular products in this series are adenosine phosphate, Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277).

For research use only. We do not sell to patients.

2-Chloro-2′-β-C-methyladenosine

2-Chloro-2′-β-C-methyladenosine Chemical Structure

CAS No. : 205171-11-5

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5 mg In-stock
10 mg In-stock
50 mg   Get quote  
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

2-Chloro-2′-β-C-methyladenosine Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2-Chloro-2′-β-C-methyladenosine is an adenosine analogue. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. The popular products in this series are adenosine phosphate, Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277)[1].

Cellular Effect
Cell Line Type Value Description References
CCRF-CEM CC50
> 100 μM
Compound: 17
Cytotoxicity against human CEM after 5 days
Cytotoxicity against human CEM after 5 days
[PMID: 21983447]
CCRF-CEM CC50
> 100 μM
Compound: 17
Cytotoxicity against human CEM cells after 6 days by trypan blue exclusion assay
Cytotoxicity against human CEM cells after 6 days by trypan blue exclusion assay
[PMID: 26819659]
Huh-7 CC50
> 10 μM
Compound: 17
Cytotoxicity against human HuH7 cells infected with HCV assessed as reduction in cellular rRNA level after 96 hrs by qRT-PCR analysis
Cytotoxicity against human HuH7 cells infected with HCV assessed as reduction in cellular rRNA level after 96 hrs by qRT-PCR analysis
[PMID: 26819659]
PBMC CC50
> 100 μM
Compound: 17
Cytotoxicity against human PBMC after 5 days
Cytotoxicity against human PBMC after 5 days
[PMID: 21983447]
PBMC CC50
> 100 μM
Compound: 17
Cytotoxicity against human PBMC after 6 days by trypan blue exclusion assay
Cytotoxicity against human PBMC after 6 days by trypan blue exclusion assay
[PMID: 26819659]
Vero CC50
> 100 μM
Compound: 17
Cytotoxicity against african green monkey Vero after 5 days
Cytotoxicity against african green monkey Vero after 5 days
[PMID: 21983447]
Vero CC50
> 100 μM
Compound: 17
Cytotoxicity against African green monkey Vero cells after 3 days by hemocytometry
Cytotoxicity against African green monkey Vero cells after 3 days by hemocytometry
[PMID: 26819659]
Molecular Weight

315.71

Formula

C11H14ClN5O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@](O)(C)[C@H](N2C=NC3=C(N)N=C(Cl)N=C32)O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (158.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1675 mL 15.8373 mL 31.6746 mL
5 mM 0.6335 mL 3.1675 mL 6.3349 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1675 mL 15.8373 mL 31.6746 mL 79.1866 mL
5 mM 0.6335 mL 3.1675 mL 6.3349 mL 15.8373 mL
10 mM 0.3167 mL 1.5837 mL 3.1675 mL 7.9187 mL
15 mM 0.2112 mL 1.0558 mL 2.1116 mL 5.2791 mL
20 mM 0.1584 mL 0.7919 mL 1.5837 mL 3.9593 mL
25 mM 0.1267 mL 0.6335 mL 1.2670 mL 3.1675 mL
30 mM 0.1056 mL 0.5279 mL 1.0558 mL 2.6396 mL
40 mM 0.0792 mL 0.3959 mL 0.7919 mL 1.9797 mL
50 mM 0.0633 mL 0.3167 mL 0.6335 mL 1.5837 mL
60 mM 0.0528 mL 0.2640 mL 0.5279 mL 1.3198 mL
80 mM 0.0396 mL 0.1980 mL 0.3959 mL 0.9898 mL
100 mM 0.0317 mL 0.1584 mL 0.3167 mL 0.7919 mL
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2-Chloro-2′-β-C-methyladenosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Chloro-2′-β-C-methyladenosine205171-11-5Nucleoside Antimetabolite/Analogmuscle vasodilatorInhibitorinhibitorinhibit

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