Cat. No.: HY-Y0694

2',4'-Dihydroxyacetophenone

(Synonyms: Resacetophenone; 1-(2,4-Dihydroxyphenyl)ethanone)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

2',4'-Dihydroxyacetophenone (Resacetophenone) is acetophenone carrying hydroxy substituents at positions 2' and 4'. 2',4'-Dihydroxyacetophenone involves in a practical CsHCO₃-mediated alkylation that efficiently provide 4-alkylated products with excellent regioselectivity, good isolated yields and a broad substrate scope. 2',4'-Dihydroxyacetophenone is a plant metabolite.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 89-84-9
Formula: C₈H₈O₃
Molecular Weight:152.15

Storage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

2',4'-Dihydroxyacetophenone Chemical Structure

CAS No.: 89-84-9

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All COX Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 25	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
LoVo	IC₅₀	97 μM Compound: 25	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
PC-3	IC₅₀	>100 μM Compound: 25	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
SK-MEL-28	IC₅₀	>100 μM Compound: 25	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
U-373MG ATCC	IC₅₀	>100 μM Compound: 25	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]

In Vitro

2',4'-Dihydroxyacetophenone (500 μM, 48 h) inhibits the transcription of COX-2 in cancer cell DLD-1 with an IC₅₀ of 500 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 89-84-9
Appearance Solid
Molecular Weight 152.15
Formula C₈H₈O₃
Color White to light brown
SMILES

CC(C1=CC=C(O)C=C1O)=O
Synonyms

Resacetophenone; 1-(2,4-Dihydroxyphenyl)ethanone
Structure Classification
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (1)

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Journal Impact Factor

iScience

GSK3β/HIF-1α signaling-dependent anti-parasite effect of Cynanchi atrati Radix. [Abstract]

2025 Dec 1;29(1):114292. PMID: 41509906

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (657.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.5725 mL

32.8625 mL

65.7250 mL

5 mM

1.3145 mL

6.5725 mL

13.1450 mL

10 mM

0.6573 mL

3.2863 mL

6.5725 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li XJ, et al. Bioactive metabolites from biotransformation of paeonol by the white-rot basidiomycete Coriolus versicolor. Nat Prod Commun. 2011 Aug;6(8):1129-30. [Content Brief]

[2]. Baughman RG, et al. 2,4-Dinitrophenylhydrazones of 2,4-dihydroxybenzaldehyde, 2,4-dihydroxyacetophenone and 2,4-dihydroxybenzophenone. Acta Crystallogr C. 2004 Feb;60(Pt 2):o103-6. [Content Brief]

[3]. Frank A, et al. Regioselective alkylation of 2,4-dihydroxybenzyaldehydes and 2,4-dihydroxyacetophenones. Tetrahedron Lett. 2022 Apr 13;95:153755. [Content Brief]

[4]. Mutoh M, et al., Suppression by flavonoids of cyclooxygenase-2 promoter-dependent transcriptional activity in colon cancer cells: structure-activity relationship. Jpn J Cancer Res. 2000 Jul;91(7):686-91. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.5725 mL	32.8625 mL	65.7250 mL	164.3126 mL
	5 mM	1.3145 mL	6.5725 mL	13.1450 mL	32.8625 mL
	10 mM	0.6573 mL	3.2863 mL	6.5725 mL	16.4313 mL
	15 mM	0.4382 mL	2.1908 mL	4.3817 mL	10.9542 mL
	20 mM	0.3286 mL	1.6431 mL	3.2863 mL	8.2156 mL
	25 mM	0.2629 mL	1.3145 mL	2.6290 mL	6.5725 mL
	30 mM	0.2191 mL	1.0954 mL	2.1908 mL	5.4771 mL
	40 mM	0.1643 mL	0.8216 mL	1.6431 mL	4.1078 mL
	50 mM	0.1315 mL	0.6573 mL	1.3145 mL	3.2863 mL
	60 mM	0.1095 mL	0.5477 mL	1.0954 mL	2.7385 mL
	80 mM	0.0822 mL	0.4108 mL	0.8216 mL	2.0539 mL
	100 mM	0.0657 mL	0.3286 mL	0.6573 mL	1.6431 mL

Keywords

2',4'-Dihydroxyacetophenone

1 Cited Publications

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2',4'-Dihydroxyacetophenone

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Publications Citing Use of MedChemExpress (MCE) 2',4'-Dihydroxyacetophenone

All Endogenous Metabolite Isoforms

All COX Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

2',4'-Dihydroxyacetophenone Related Classifications

HY-Y0694 Related Classifications