1. MAPK/ERK Pathway
    Stem Cell/Wnt
    NF-κB
  2. ERK
    NF-κB
  3. 2,5-Dihydroxyacetophenone

2,5-Dihydroxyacetophenone 

Cat. No.: HY-W001174 Purity: 99.56%
Handling Instructions

2,5-Dihydroxyacetophenone, isolated from Rehmanniae Radix Preparata, inhibits the production of inflammatory mediators in activated macrophages by blocking the ERK1/2 and NF-κB signaling pathways.

For research use only. We do not sell to patients.

2,5-Dihydroxyacetophenone Chemical Structure

2,5-Dihydroxyacetophenone Chemical Structure

CAS No. : 490-78-8

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 55 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

2,5-Dihydroxyacetophenone, isolated from Rehmanniae Radix Preparata, inhibits the production of inflammatory mediators in activated macrophages by blocking the ERK1/2 and NF-κB signaling pathways[1].

IC50 & Target[1]

ERK1

 

ERK2

 

NF-κB

 

In Vitro

2,5-Dihydroxyacetophenone significantly inhibits NO production via the suppression of iNOS expression and significantly decreases levels of the pro-inflammatory cytokines TNF-α and IL-6 via the down-regulation of their mRNA expression in LPS-stimulated RAW264.7 cells. 2,5-Dihydroxyacetophenone potently inhibits the phosphorylation of extracellular signal-related kinase (ERK) 1/2 and the nuclear translocation of nuclear factor-κB (NF-κB) p65 in LPS-stimulated cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

152.15

Formula

C₈H₈O₃

CAS No.

490-78-8

SMILES

CC(C1=CC(O)=CC=C1O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (657.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5725 mL 32.8623 mL 65.7246 mL
5 mM 1.3145 mL 6.5725 mL 13.1449 mL
10 mM 0.6572 mL 3.2862 mL 6.5725 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (16.43 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

2,5-DihydroxyacetophenoneERKNF-κBExtracellular signal regulated kinasesNuclear factor-κBNuclear factor-kappaBRehmanniaeRadixPreparatainflammatorymediatorsmacrophagesNOiNOSTNF-αIL-6mRNALPS-stimulatedRAW264.7p65Inhibitorinhibitorinhibit

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Product Name:
2,5-Dihydroxyacetophenone
Cat. No.:
HY-W001174
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