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Cat. No.: HY-183022

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3-Azidopropyl-1-maleimide

1-(3-Azidopropyl)-1H-pyrrole-2,5-dione is a maleimide derivative with a 3-azidopropyl substituent, used to introduce an azide functional group to bovine serum albumin (BSA). 1-(3-Azidopropyl)-1H-pyrrole-2,5-dione reacts with the free, solvent-accessible thiol group of BSA to produce azide-functionalized BSA, which is used in click chemistry to form a TLL-BSA hetero-dimer.

For research use only. We do not sell to patients.

CAS No.: 896465-45-5
Formula: C₇H₈N₄O₂
Molecular Weight:180.17

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

3-Azidopropyl-1-maleimide Chemical Structure

CAS No.: 896465-45-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Description

Chemical Information

CAS No. 896465-45-5
Molecular Weight 180.17
Formula C₇H₈N₄O₂
SMILES

[N-]=[N+]=NCCCN1C(=O)C=CC1=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hatzakis NS, et al. Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer. Chem Commun (Camb). 2006;(19):2012-2014. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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