Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer

  • Chem Commun (Camb). 2006 May 21:(19):2012-4. doi: 10.1039/b516551b.
Nikos S Hatzakis  1 ,  Hans Engelkamp ,  Kelly Velonia ,  Johan Hofkens ,  Peter C M Christianen ,  Allan Svendsen ,  Shamkantr A Patkar ,  Jesper Vind ,  Jan C Maan ,  Alan E Rowan ,  Roeland J M Nolte
Affiliations
  • 1. Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands.
PMID: 16767259 DOI: 10.1039/b516551b
Abstract

Click chemistry is used to construct a novel lipase-BSA hetero-dimer, in which the latter protein acts as a foot enabling the anchoring of the enzyme onto the surface for single enzyme studies.

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