Art. -Nr.: HY-N7661

4β-Hydroxywithanolide E

Name: 4β-Hydroxywithanolide E
Brand: MedChemExpress
SKU: HY-N7661
Price: 680.00 USD
Availability: InStock
Rating: 5 (1 reviews)

Kundenbewertung

Based on 1 Customer Validation

4β-Hydroxywithanolide E, isolated from Physalis peruviana L., inhibits adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion. 4β-Hydroxywithanolide E is an adipogenesis inhibitor and inhibits PPARγ, C/EBPα, and the adipocyte-specific molecule aP2 mRNA expression.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit : 98%
CAS. Nr.: 54334-04-2
Formel: C₂₈H₃₈O₈
Molecular Weight:502.60

Speicherung:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

4β-Hydroxywithanolide E Chemical Structure

CAS. Nr.: 54334-04-2

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
Chemical Information
Protokoll (6)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle PPAR Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
ACHN	IC₅₀	>2 μM Compound: 12	Cytotoxicity against human ACHN cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human ACHN cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
ACHN	IC₅₀	563 nM Compound: 5	Sensitization of TRAIL-induced apoptosis in human ACHN cells assessed as reduction in cell viability preincubated for 2 hrs followed by addition of TRAIL measured after 18 hrs by MTS assay Sensitization of TRAIL-induced apoptosis in human ACHN cells assessed as reduction in cell viability preincubated for 2 hrs followed by addition of TRAIL measured after 18 hrs by MTS assay	[PMID: 28257574]
CWR22R	IC₅₀	0.09 μM Compound: 12	Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
HEK293	IC₅₀	0.04 μM Compound: 4	Inhibition of TNFalpha-activated nuclear factor-kappa B activity (unknown origin) expressed in HEK293 cells incubated for 6 hrs by luciferase reporter gene based assay Inhibition of TNFalpha-activated nuclear factor-kappa B activity (unknown origin) expressed in HEK293 cells incubated for 6 hrs by luciferase reporter gene based assay	[PMID: 27210437]
HFF	IC₅₀	>2 μM Compound: 12	Cytotoxicity against HFF cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against HFF cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
LNCaP	IC₅₀	0.16 μM Compound: 12	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
M14	IC₅₀	>2 μM Compound: 12	Cytotoxicity against human M14 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human M14 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
MCF7	GI₅₀	1 μM Compound: 2	Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 48 hrs by SRB assay Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 48 hrs by SRB assay	[PMID: 20438092]
MDA-MB-231	GI₅₀	2.1 μM Compound: 2	Antiproliferative activity against estrogen receptor deficient human MDA-MB-231 cells after 48 hrs by SRB assay Antiproliferative activity against estrogen receptor deficient human MDA-MB-231 cells after 48 hrs by SRB assay	[PMID: 20438092]
RAW264.7	IC₅₀	0.32 μM Compound: 4	Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay	[PMID: 27210437]
RAW264.7	IC₅₀	1.8 μM Compound: 1	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 30 mins followed by LPS challenge and measured after 18 hrs by Griess assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 30 mins followed by LPS challenge and measured after 18 hrs by Griess assay	[PMID: 30649869]
SK-BR-3	GI₅₀	1.7 μM Compound: 2	Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 48 hrs by SRB assay Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 48 hrs by SRB assay	[PMID: 20438092]
SK-MEL-28	IC₅₀	>2 μM Compound: 12	Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 28617598]
T47D	GI₅₀	2.2 μM Compound: 2	Antiproliferative activity against human T47D cells expressing estrogen receptor after 48 hrs by SRB assay Antiproliferative activity against human T47D cells expressing estrogen receptor after 48 hrs by SRB assay	[PMID: 20438092]

Chemical Information

CAS. Nr. 54334-04-2
Appearance Solid
Molecular Weight 502.60
Formel C₂₈H₃₈O₈
Color White to light yellow
SMILES

C[C@]1(C2=O)[C@@]3([C@H](C=C2)O)[C@](C[C@]([C@@]4(CC[C@](O)5[C@@](O)([C@@](OC6=O)([H])CC(C)=C6C)C)O)([H])[C@]1([H])CC[C@@]45C)([H])O3
Structure Classification
- Steroids
Initial Source
- Plants
- Solanaceae
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Protokoll

All Molecular Biology Cell Biology Histomorphology

RT-PCR
Reverse transcription technology uses RNA as a template to synthesize DNA. RT-PCR is simple, specific and sensitive, and can be used to detect gene expression levels and expression differences in cells; detect RNA virus content; clone cDNA sequences of specific genes.
3T3-L1 preadipocyte-to-adipocyte differentiation
3T3-L1 preadipocytes are induced to differentiate after growth arrest using adipogenic media containing insulin, dexamethasone, and IBMX; differentiation is assessed by lipid-droplet accumulation, triglyceride increase, Oil Red O staining, and adipocyte-marker induction such as PPARγ and C/EBPα.
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Large-size fat particle sorting
Large-size fat particle sorting is widely used to isolate cells up to 200 μm in diameter. Single-cell flow sorting will allow greater insight into adipocyte heterogeneity by identifying gene expression, protein composition, and metabolic signatures at the single-cell level.
Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Momochika Kumagai, et al. 4β-Hydroxywithanolide E and withanolide E from Physalis peruviana L. inhibit adipocyte differentiation of 3T3-L1 cells through modulation of mitotic clonal expansion. J Nat Med. 2021 Jan;75(1):232-239. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

4β-Hydroxywithanolide E

Alle PPAR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Beschreibung

Cellular Effect

Chemical Information

Protokoll

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Bioz

Keywords

Your Recently Viewed Products

4β-Hydroxywithanolide E

Alle PPAR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Beschreibung

Cellular Effect

Chemical Information

Protokoll

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs