Cat. No.: HY-N6856

4-Hydroxycoumarin

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4-Hydroxycoumarin is an orally active coumarin derivative, one of the most versatile heterocyclic scaffolds, often used in the synthesis of various organic compounds. 4-Hydroxycoumarin possesses both electrophilic and nucleophilic properties. 4-Hydroxycoumarin is an HIV protease inhibitor and tyrosine kinase inhibitor. 4-Hydroxycoumarin has anti-inflammatory, antibacterial and anti-tumor effects.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 1076-38-6
Formula: C₉H₆O₃
Molecular Weight:162.14

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

4-Hydroxycoumarin Chemical Structure

CAS No.: 1076-38-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	70.74 μM Compound: 222-1	Cytotoxicity against mouse B16F10 cells assessed as reduction in cell viability Cytotoxicity against mouse B16F10 cells assessed as reduction in cell viability	[PMID: 30594028]
EA.hy 926	EC₅₀	>200 μM Compound: 3, 4-HC	Cytotoxicity against human EAhy926 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human EAhy926 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24721833]
EA.hy 926	EC₅₀	>200 μM Compound: 3, 4-HC	Cytotoxicity against human EAhy926 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human EAhy926 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 24721833]
LNCaP	EC₅₀	100.51 μM Compound: 3, 4-HC	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24721833]
LNCaP	EC₅₀	120.27 μM Compound: 3, 4-HC	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 24721833]
MCF7	EC₅₀	>200 μM Compound: 3, 4-HC	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24721833]
MCF7	EC₅₀	199.82 μM Compound: 3, 4-HC	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 24721833]
MCF7	GI₅₀	95.34 μM Compound: 4a	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 28041801]
MDA-MB-231	EC₅₀	>200 μM Compound: 3, 4-HC	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24721833]
MDA-MB-231	EC₅₀	100.26 μM Compound: 3, 4-HC	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 24721833]
MDA-MB-231	IC₅₀	>100 μM Compound: 21.4	Antiproliferative activity against human MDA-MB-231 cells incubated for 96 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 96 hrs by MTT assay	[PMID: 38340509]
MDA-MB-231	IC₅₀	>=100 μM Compound: Hydroxycoumarin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 72 hrs under hypoxia condition by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 72 hrs under hypoxia condition by MTT assay	[PMID: 38283214]
MDA-MB-231	IC₅₀	>=100 μM Compound: Hydroxycoumarin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 72 hrs under normoxia condition by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 72 hrs under normoxia condition by MTT assay	[PMID: 38283214]
M-HeLa	IC₅₀	>=14.7 μM Compound: 102a	Anticancer activity against human HelaM cells assessed as cell growth inhibition measured after 24 hrs by DAPI and propidium iodide staining based assay Anticancer activity against human HelaM cells assessed as cell growth inhibition measured after 24 hrs by DAPI and propidium iodide staining based assay	[PMID: 36481599]
PC-3	EC₅₀	150.46 μM Compound: 3, 4-HC	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 24721833]
PC-3	EC₅₀	173.31 μM Compound: 3, 4-HC	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 24721833]
SK-MEL-28	IC₅₀	1.3 μM Compound: 9	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	2.1 μM Compound: 9	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
TK-10	GI₅₀	>100 μM Compound: 4a	Cytotoxicity against human TK10 cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human TK10 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 28041801]
U-937	EC₅₀	>200 μM Compound: 3, 4-HC	Cytotoxicity against human U937 cells assessed as reduction in cell viability after 48 hrs by Alamar blue assay Cytotoxicity against human U937 cells assessed as reduction in cell viability after 48 hrs by Alamar blue assay	[PMID: 24721833]
U-937	EC₅₀	164.12 μM Compound: 3, 4-HC	Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by Alamar blue assay Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by Alamar blue assay	[PMID: 24721833]
UACC-62	GI₅₀	83.6 μM Compound: 4a	Cytotoxicity against human UACC62 cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human UACC62 cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 28041801]

In Vitro

4-Hydroxycoumarin (0, 50, 160, 500 μM, 24 h) disrupts the actin cytoskeleton in B16-F10 melanoma cells but not in B82 fibroblasts. However, they can reduce their adhesion and motility to extracellular matrix proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

B16-F10, B82
Concentration:

0, 50, 160, 500 μM
Incubation Time:

24 h
Result:

Had no appreciable effect on cell viability.

Western Blot Analysis ^[2]

Cell Line:

B16-F10
Concentration:

0, 50, 160, 500 μM
Incubation Time:

24 h
Result:

Reduced the adhesion to ECM proteins and the tyrosine phosphorylation of several proteins in a concentration-dependent manner.
Inhibited the migration at 500 μM.

In Vivo

4-Hydroxycoumarin (10, 20 or 40 mg/kg/day, oral gavage) can effectively inhibit tumor growth and improve survival rate in melanoma mice^[3].
4-Hydroxycoumarin (5, 10, 25, 50 mg/kg/ day, Oral gavage) can significantly reduce symptoms in a rat model of trinitrobenzene sulfonic acid-induced colitis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse melanoma model^[3]
Dosage:

10, 20 or 40 mg/kg
Administration:

i.g.
Result:

Reduced >85% of the number of pulmonary tumors.
Diminished the tumor size from day 22 and increased survival time at 10 mg/kg/day.

Animal Model:

Rat colitis model ^[4]
Dosage:

5, 10, 25, 50 mg/kg
Administration:

p.o.
Result:

Reduced damage score and extension of the lesion at doses of 10 and 25 mg/kg.
Counteracted GSH content and reduced AP activity at a dose of 5 mg/kg.
Showed a good recovery of the intestinal cytoarchitecture at doses of 5 and 25 mg/kg.

Chemical Information

CAS No. 1076-38-6
Appearance Solid
Molecular Weight 162.14
Formula C₉H₆O₃
Color White to off-white
SMILES

O=C1C=C(O)C2=CC=CC=C2O1
Structure Classification
- Phenylpropanoids
- Coumarins
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (616.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.1674 mL

30.8368 mL

61.6736 mL

5 mM

1.2335 mL

6.1674 mL

12.3347 mL

10 mM

0.6167 mL

3.0837 mL

6.1674 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (12.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (12.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (12.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mohammadi Ziarani G, et al. The molecular diversity scope of 4-hydroxycoumarin in the synthesis of heterocyclic compounds via multicomponent reactions. Mol Divers. 2019 Jan 29. doi: 10.1007/s11030-019-09918-7. [Content Brief]

[2]. Velasco-Velázquez MA, et al. 4-Hydroxycoumarin disorganizes the actin cytoskeleton in B16-F10 melanoma cells but not in B82 fibroblasts, decreasing their adhesion to extracellular matrix proteins and motility. Cancer Lett. 2003 Aug 20;198(2):179-86. [Content Brief]

[3]. Salinas-Jazmín N, et al. Antimetastatic, antineoplastic, and toxic effects of 4-hydroxycoumarin in a preclinical mouse melanoma model. Cancer Chemother Pharmacol. 2010 Apr;65(5):931-40. [Content Brief]

[4]. Luchini AC, et al. Intestinal anti-inflammatory activity of coumarin and 4-hydroxycoumarin in the trinitrobenzenesulphonic acid model of rat colitis. Biol Pharm Bull. 2008 Jul;31(7):1343-50. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.1674 mL	30.8368 mL	61.6736 mL	154.1839 mL
	5 mM	1.2335 mL	6.1674 mL	12.3347 mL	30.8368 mL
	10 mM	0.6167 mL	3.0837 mL	6.1674 mL	15.4184 mL
	15 mM	0.4112 mL	2.0558 mL	4.1116 mL	10.2789 mL
	20 mM	0.3084 mL	1.5418 mL	3.0837 mL	7.7092 mL
	25 mM	0.2467 mL	1.2335 mL	2.4669 mL	6.1674 mL
	30 mM	0.2056 mL	1.0279 mL	2.0558 mL	5.1395 mL
	40 mM	0.1542 mL	0.7709 mL	1.5418 mL	3.8546 mL
	50 mM	0.1233 mL	0.6167 mL	1.2335 mL	3.0837 mL
	60 mM	0.1028 mL	0.5139 mL	1.0279 mL	2.5697 mL
	80 mM	0.0771 mL	0.3855 mL	0.7709 mL	1.9273 mL
	100 mM	0.0617 mL	0.3084 mL	0.6167 mL	1.5418 mL

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4-Hydroxycoumarin

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All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

4-Hydroxycoumarin

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

Kinase Inhibitor Library

Metabolism/Protease Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antibacterial Compound Library

Medicine Food Homology Compound Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Human Metabolite Library

Mitochondrial Toxicity Compound Library

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Posttranslational Modification Library

Mongolian Medicine Compound Library

Mass Spectrometry Natural Product Library

Drug Fragment Library

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

4-Hydroxycoumarin Related Classifications

HY-N6856 Related Classifications