Acetoacetic acid sodium

Name: Acetoacetic acid sodium
Brand: MedChemExpress
SKU: HY-112540B
Rating: 4.5 (2 reviews)

Cat. No.: HY-112540B Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Acetoacetic acid sodium is a metabolite of non-esterified fatty acids, involved in the development of human diabetes. Acetoacetic acid sodium induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes.

For research use only. We do not sell to patients.

Acetoacetic acid sodium Chemical Structure

CAS No. : 623-58-5

Size	Price	Stock	Quantity
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Acetoacetic acid sodium:

Acetoacetic acid lithium In-stock
Acetoacetic acid Get quote

2 Publications Citing Use of MCE Acetoacetic acid sodium

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Acetoacetic acid sodium induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes^[1].
? Acetoacetic acid (0.6, 2.4, 4.8 mM) increases malondialdehyde (MDA) content in high-dose group (GH) group, decreases mRNA expression of Mn SOD, Cu/Zn SOD, and glutathione peroxidase (GSH-Px) in all groups, lowers catalase (CAT) mRNA expression in medial-dose group (GM), and GH groups. In addition, Acetoacetic acid down-regulates the mRNA expression of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and low density lipoprotein receptor (LDLR). Thus, VLDL assembly is decreased andtriglyceride (TG) accumulation is increased in these bovine hepatocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

124.07

Formula

C₄H₅NaO₃

CAS No.

623-58-5

Appearance

Solid

Color

White to off-white

SMILES

CC(CC(O[Na])=O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : ≥ 125 mg/mL (1007.50 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.0600 mL	40.2998 mL	80.5997 mL
5 mM	1.6120 mL	8.0600 mL	16.1199 mL
10 mM	0.8060 mL	4.0300 mL	8.0600 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.04 mg/mL (8.38 mM); Clear solution

This protocol yields a clear solution of ≥ 1.04 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.4 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.04 mg/mL (8.38 mM); Clear solution

This protocol yields a clear solution of ≥ 1.04 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.4 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (806.00 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (189 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Shi X, et al. Acetoacetic acid induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes. J Dairy Res. 2016 Nov;83(4):442-446. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	8.0600 mL	40.2998 mL	80.5997 mL	201.4992 mL
	5 mM	1.6120 mL	8.0600 mL	16.1199 mL	40.2998 mL
	10 mM	0.8060 mL	4.0300 mL	8.0600 mL	20.1499 mL
	15 mM	0.5373 mL	2.6867 mL	5.3733 mL	13.4333 mL
	20 mM	0.4030 mL	2.0150 mL	4.0300 mL	10.0750 mL
	25 mM	0.3224 mL	1.6120 mL	3.2240 mL	8.0600 mL
	30 mM	0.2687 mL	1.3433 mL	2.6867 mL	6.7166 mL
	40 mM	0.2015 mL	1.0075 mL	2.0150 mL	5.0375 mL
	50 mM	0.1612 mL	0.8060 mL	1.6120 mL	4.0300 mL
	60 mM	0.1343 mL	0.6717 mL	1.3433 mL	3.3583 mL
	80 mM	0.1007 mL	0.5037 mL	1.0075 mL	2.5187 mL
	100 mM	0.0806 mL	0.4030 mL	0.8060 mL	2.0150 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.