Naloxone hydrochloride
Based on 8 publication(s) in Google Scholar
Naloxone hydrochloride is an antagonist of Opioid receptor. Naloxone hydrochloride alleviates opioid-overdose-induced respiratory depression. Naloxone hydrochloride may cause pulmonary edema and cardiac arrhythmias.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 357-08-4
- Formula: C19H22ClNO4
- Molecular Weight:363.84
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Naloxone hydrochloride
More- Nature. 2025 Dec;648(8094):755-763. [Abstract]
- Nat Methods. 2026 May;23(5):1011-1023. [Abstract]
- Nat Commun. 2025 Aug 1;16(1):7074. [Abstract]
- Cell Rep. 2020 Mar 17;30(11):3625-3631.e6. [Abstract]
- Br J Pharmacol. 2025 Jul 21. [Abstract]
- J Med Chem. 2026 Mar 5;69(6):6508-6527.
- Eur J Pain. 2017 May;21(5):804-814. [Abstract]
- bioRxiv. 2026 Jun 3.
Customer Validation & Images
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Others
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Cell Proliferation/Viability Assay
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Others
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Cell Proliferation/Viability Assay
All Opioid Receptor Isoforms
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Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 357-08-4
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Appearance Solid
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Molecular Weight 363.84
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Formula C19H22ClNO4
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Color White to off-white
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SMILES
OC1=C(O2)C([C@]([C@]2([H])C(CC3)=O)(CCN4CC=C)[C@]3(O)[C@H]4C5)=C5C=C1.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (8)
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Journal Impact Factor
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Most Recent
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Nature
2025 Dec;648(8094):755-763. PMID: 41193810 -
Nat Methods
Single-molecule localization and diffusivity microscopy reveals dynamic biomolecular organization in living cells. [Abstract]2026 May;23(5):1011-1023. PMID: 42050111 -
Nat Commun
Neuropeptide Y neurons mediate opioid-induced itch by disinhibiting GRP-GRPR microcircuits in the spinal cord. [Abstract]2025 Aug 1;16(1):7074. PMID: 40750771
Naloxone hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 1;16(1):7074. [Abstract]
Heatmaps shows the calcium signals within the 10-25 min after drugs injection (i.t., 0.3 nmol Morphine, 10 nmol Naloxone + 0.3 nmol Morphine, and 10 μg NPY + 0.3 nmol Morphine), n = 5 mice per group.
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Cell Rep
Morphine and Naloxone Facilitate Neural Stem Cells Proliferation via a TET1-Dependent and Receptor-Independent Pathway. [Abstract]2020 Mar 17;30(11):3625-3631.e6. PMID: 32187535
Naloxone hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Rep. 2020 Mar 17;30(11):3625-3631.e6. [Abstract]
NSCs were treated with different concentrations of Naloxone, Morphine, Naloxone and Morphine (Mor+Nal, 1:1), or Nal-M. Cell numbers were determined on day 3.
Naloxone hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Rep. 2020 Mar 17;30(11):3625-3631.e6. [Abstract]
Neural stem cells (NSCs) were treated with 1 μM Naloxone or Morphine. The contents of 5mC and 5hmC in NSCs (day 3) were determined by high-spot Western blot assay.
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Br J Pharmacol
Semax peptide targets the μ opioid receptor gene Oprm1 to promote deubiquitination and functional recovery after spinal cord injury in female mice. [Abstract]2025 Jul 21. PMID: 40692165 -
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Eur J Pain
Endocannabinoid activation of CB1 receptors contributes to long-lasting reversal of neuropathic pain by repetitive spinal cord stimulation. [Abstract]2017 May;21(5):804-814. PMID: 28107590
Naloxone hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Pain. 2017 May;21(5):804-814. [Abstract]
The SCS1 (early SCS)-mediated increase in the ipsilateral:contralateral PWT ratio (compared to pre-SCS) is significantly reduced by the opioid receptor antagonist, Naloxone, which is administered 10 min before SCS1.
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Solvent & Solubility
H2O : 62.5 mg/mL (171.78 mM; Need ultrasonic)
DMSO : ≥ 30 mg/mL (82.45 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
Young adult Wistar rats are used in the assay: females for experiments l and 3 (age 50-70 days, weight 100-185 g) and males for experiments 2 and 4 (age 51-69 days, weight 140-200 g). The same apparatus is used for both: a 50×25×25 cm box made of plywood with a frontal glass wall, whose floor consists of 1 mm-caliber bronze bars spaced l0 mm apart. In task l, a 5 cm high, 25×25 cm wooden platform is introduced into the box so as to cover the left half of the grid. Rats are held gently by their bodies and loared onto the platform facing the rear left corner, at which time a quartz chronometer is activated. Time is counted until the animals stepped down from the platform and placed their four paws on the grid, upon which a 0.5 mA, 60 Hz footshock is continuously delivered until the animals return to the platform. In task 2 the platform is only 7 cm wide and covered only the leftmost seven bars of the grid; the footshock is of only 0.3 mA and is delivered in 0.4 msec pulses once every 2 sec until the animals climb back onto the platform. Immediately after training in each task the animals are withdrawn from the box injected i.p. with one of the following: saline (1.0 mL/kg), ACTH1-24 (0.2 or 2.0 μg/kg), epinephrine HCl (5.0 or 50.0 μg/kg), human β-endorphin (0.1 or 1.0 μg/kg), naloxone HCl (0.4 mg/kg), or ACTH or epinephrine given together with either β-endorphin or naloxone. All drugs are dissolved in saline to an injection volume of 1.0 mL/kg. Twenty-four hr after training the animals again are placed on the platform (the wide one for task 1, experiments 1 and 2, and the narrow one for task 2, experiments 3 and 4), and their latency to stepdown onto the grid again is measured as in the training session. A ceiling of 180 sec is imposed on this measure.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. McIntosh TK, et al. Beneficial effect of the nonselective opiate antagonist naloxone hydrochloride and the thyrotropin-releasing hormone (TRH) analog YM-14673 on long-term neurobehavioral outcome following experimental brain injury in the rat. J Neurotrau [Content Brief]
[2]. Sun L, et al. Endocannabinoid activation of CB1 receptors contributes to long-lasting reversal of neuropathic pain by repetitive spinal cord stimulation. Eur J Pain. 2017 May;21(5):804-814. [Content Brief]
[3]. Izquierdo I, et al. Effect of ACTH, epinephrine, beta-endorphin, naloxone, and of the combination of naloxone or beta-endorphinwith ACTH or epinephrine on memory consolidation. Psychoneuroendocrinology. 1983;8(1):81-7. [Content Brief]
[4]. Soteropoulos GC, et al. Neuromuscular effects of morphine and naloxone. J Pharmacol Exp Ther. 1973 Jan;184(1):136-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.7485 mL | 13.7423 mL | 27.4846 mL | 68.7115 mL |
| 5 mM | 0.5497 mL | 2.7485 mL | 5.4969 mL | 13.7423 mL | |
| 10 mM | 0.2748 mL | 1.3742 mL | 2.7485 mL | 6.8712 mL | |
| 15 mM | 0.1832 mL | 0.9162 mL | 1.8323 mL | 4.5808 mL | |
| 20 mM | 0.1374 mL | 0.6871 mL | 1.3742 mL | 3.4356 mL | |
| 25 mM | 0.1099 mL | 0.5497 mL | 1.0994 mL | 2.7485 mL | |
| 30 mM | 0.0916 mL | 0.4581 mL | 0.9162 mL | 2.2904 mL | |
| 40 mM | 0.0687 mL | 0.3436 mL | 0.6871 mL | 1.7178 mL | |
| 50 mM | 0.0550 mL | 0.2748 mL | 0.5497 mL | 1.3742 mL | |
| 60 mM | 0.0458 mL | 0.2290 mL | 0.4581 mL | 1.1452 mL | |
| 80 mM | 0.0344 mL | 0.1718 mL | 0.3436 mL | 0.8589 mL | |
| H2O | 100 mM | 0.0275 mL | 0.1374 mL | 0.2748 mL | 0.6871 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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