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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2969
    CYDA
    CYDA is a colorimetric probe based on cyanine dyes, which enables highly sensitive and selective detection of Cu²⁺. CYDA can be used for urine copper detection in Wilson's disease.
    CYDA
  • HY-D1385
    JF526–Pepstatin A TFA
    JF526–Pepstatin A TFA is a fluorescent dye that can be used for lysosomal staining in live cells. The excitation maximum is 530 nm and the emission maximum is 549 nm.
    JF526–Pepstatin A TFA
  • HY-NP064
    Bauhinia Purpurea Lectin
    Bauhinia Purpurea Lectin (BPL) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Bauhinia Purpurea Lectin (BPL) is a biological material or organic compound that can be used in life science research.
    Bauhinia Purpurea Lectin
  • HY-D2073
    ATTO 680 NHS ester
    ATTO 680 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 680 NHS ester is an NHS ester derivative of ATTO 680 that can be used to label proteins or antibodies.
    ATTO 680 NHS ester
  • HY-D0124
    Phthalimidylbenzenesulfonyl chloride
    Phthalimidylbenzenesulfonyl chloride is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    Phthalimidylbenzenesulfonyl chloride
  • HY-D3054
    5(6)-Rhodamine 6G amine
    5(6)-Rhodamine 6G amine is a primary amine fluorescent reagent based on Rhodamine 6G (HY-D0309). Primary amines can be covalently linked with various functional groups such as carboxylic acids, active esters (e.g., NHS esters), and isothiocyanates to achieve labeling (Ex/Em = 526/547 nm).
    5(6)-Rhodamine 6G amine
  • HY-D2836E
    FITC-HA (MW 10000)
    FITC-HA (MW 10000) is hyaluronic acid (HA) (HY-B0633A) labeled with FITC (HY-66019). FITC-HA retains the ability of HA to bind to receptors (such as CD44) and form extracellular matrices, while it can be detected by fluorescence microscopy or flow cytometry for tracing the localization, binding, internalization and metabolic pathways of HA in cells, tissues or living organisms (Ex/Em ≈ 490/520 nM).
    FITC-HA (MW 10000)
  • HY-D2533
    Cy5-PEG2000-N3
    Cy5-PEG2000-N3 is a Cy5 (HY-D0821)-azide fluorescent dye used to label for protein and nucleic acid. Cy5-PEG2000-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy5-PEG2000-N3
  • HY-D1447
    Fluo-3FF pentapotassium
    Fluo-3FF pentapotassium is a cell-impermeant fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds=42 and 0.4 μM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.
    Fluo-3FF pentapotassium
  • HY-W283359
    4-Methylumbelliferyl β-D-ribofuranoside
    4-Methylumbelliferyl β-D-ribofuranoside is a fluorogenic substrate of β-ribosidase.
    4-Methylumbelliferyl β-D-ribofuranoside
  • HY-W440914A
    DSPE-PEG400-FITC
    DSPE-PEG400-FITC is an amphiphile PEG polymer which has unsaturated lipid tails. DSPE-PEG400-FITC can form micelles in an aqueous solution and can be used to prepare liposome/nano particles for drug nanocarriers.
    DSPE-PEG400-FITC
  • HY-D1979
    ATTO 725 NHS-Ester
    ATTO 725 NHS-Ester is an activated ester derivative of ATTO 725, which can directly label proteins or antibodies. The maximum excitation/emission wavelength: 728/751 nm.
    ATTO 725 NHS-Ester
  • HY-D1850
    Sulfo-Cy7.5 azide
    Sulfo-Cy7.5 azide is a Cyanine 7.5 (Cy7.5) (HY-D0926) dye derivative with azide and sulfonate functional groups. The sulfonate ion increases the water solubility of the compound, making it suitable for use in aqueous solutions. Cy7.5 is a near-infrared fluorescent dye commonly used for biolabeling and cell imaging. The azide group of Sulfo-Cy7.5 azide can react chemically with molecules containing alkyne functionality, such as alkyne or cyclooctyne, to form covalent bonds. Therefore, Sulfo-Cy7.5 azide can bind to biomolecules such as proteins and antibodies to track their location and dynamic changes in biological samples. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    Sulfo-Cy7.5 azide
  • HY-D2079
    ATTO 665 azide
    ATTO 665 azide is an azide derivative of ATTO 665 with excitation/emission maxima at 663/680 nm.
    ATTO 665 azide
  • HY-D1268
    Seph-PAO
    Seph-PAO is the modified PAO attached a sepharose fluorophore and is used to detect the thioredoxin reductase (TrxR).
    Seph-PAO
  • HY-148828E
    Cy3 labled LSP-GR3 sodium
    Cy3 labled LSP-GR3 sodium is a Cy3 labled LSP-GR3 sodium.
    Cy3 labled LSP-GR3 sodium
  • HY-170603
    PRMT5-targeted fluorescent ligand-1
    PRMT5-targeted fluorescent ligand-1 (Compound 7) is a PRMT5-targeted fluorescent ligand, that inhibits PRMT5 with an IC50 of 29.39 nM. PRMT5-targeted fluorescent ligand-1 exhibits cell imaging activity, that exhibits good fluorescence signal in MCF-7 with an IC50 of 29 nM. PRMT5-targeted fluorescent ligand-1 induces apoptosis. PRMT5-targeted fluorescent ligand-1 exhibits a maximal excitation wavelength of 438 nm and a maximal emission wavelength of 550 nm.
    PRMT5-targeted fluorescent ligand-1
  • HY-138658
    Janelia Fluor® 526, SE
    Janelia Fluor 526, SE (JF526,SE) is a fluorogenic yellow fluorescent dye that contains NHS ester group. JF526 is a versatile scaffold for fluorogenic ligands, including labels for genetically encoded self-labeling protein tags and stains for endogenous structures. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    Janelia Fluor® 526, SE
  • HY-141286
    TAMRA-PEG4-methyltetrazine
    TAMRA-PEG4-methyltetrazine is a dye derivative of TAMRA (HY-135640) containing 4 PEG units. TAMRA-PEG4-methyltetrazine contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
    TAMRA-PEG4-methyltetrazine
  • HY-D3239
    AF405 NHS Ester
    AF405 NHS Ester (AF405) is a Fluorescent probe substrate, as well as a substrate for OATP1B1, OATP1B3 and OATP2B1.
    AF405 NHS Ester
Cat. No. Product Name / Synonyms Application Reactivity