(R)-(-)-Rolipram
Based on 2 publication(s) in Google Scholar
(R)-(-)-Rolipram is the R-enantiomer of Rolipram. Rolipram is a selective inhibitor of phosphodiesterases PDE4 with IC50 of 3 nM, 130 nM and 240 nM for PDE4A, PDE4B, and PDE4D, respectively.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 85416-75-7
- Formula: C16H21NO3
- Molecular Weight:275.34
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) (R)-(-)-Rolipram
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Customer Validation & Images
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WB
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | CC50 |
>10 μM
Compound: 13a
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Cytotoxicity against HEK293T cells
Cytotoxicity against HEK293T cells
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[PMID: 16632349] |
| Sf9 | IC50 |
231 nM
Compound: CHEMBL430893
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Inhibition of human N-terminal His6-tagged C-terminal FLAG-tagged PDE4B2 expressed in baculovirus infected sf9 cells using cAMP as substrate by microplate reader analysis
Inhibition of human N-terminal His6-tagged C-terminal FLAG-tagged PDE4B2 expressed in baculovirus infected sf9 cells using cAMP as substrate by microplate reader analysis
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[PMID: 26908025] |
The increase of cAMP synthesis with the adenylate cyclase activator forskolin or the decrease of cAMP hydrolysis by the phosphodiesterase inhibitors Isobutylmethylxanthine (IBMX) and (R)-(-)-Rolipram (MedChem Express) suppresses caspase-1 cleavage and IL-1β secretion in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 85416-75-7
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Appearance Solid
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Molecular Weight 275.34
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Formula C16H21NO3
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Color Off-white to light yellow
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SMILES
O=C1NC[C@@H](C2=CC=C(OC)C(OC3CCCC3)=C2)C1
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Synonyms
(R)-Rolipram; (-)-Rolipram
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Immunity
Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome. [Abstract]2016 Oct 18;45(4):944. PMID: 27760343
(R)-(-)-Rolipram purchased from MedChemExpress. Usage Cited in: Immunity. 2016 Oct 18;45(4):944. [Abstract]
LPS-primed BMDMs are treated with different doses of (R)-(-)-Rolipram, and then stimulated with Nigericin for 45 min. Supernatants (SN) and cell extracts (Lysate) are analyzed by immunoblotting. The phosphodiesterase inhibitors isobutylmethylxanthine (IBMX) and (R)-(-)-rolipram suppresses caspase-1 cleavage and IL-1β secretion in a dose-dependent manner.
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Solvent & Solubility
DMSO : 100 mg/mL (363.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6319 mL | 18.1594 mL | 36.3187 mL | 90.7968 mL |
| 5 mM | 0.7264 mL | 3.6319 mL | 7.2637 mL | 18.1594 mL | |
| 10 mM | 0.3632 mL | 1.8159 mL | 3.6319 mL | 9.0797 mL | |
| 15 mM | 0.2421 mL | 1.2106 mL | 2.4212 mL | 6.0531 mL | |
| 20 mM | 0.1816 mL | 0.9080 mL | 1.8159 mL | 4.5398 mL | |
| 25 mM | 0.1453 mL | 0.7264 mL | 1.4527 mL | 3.6319 mL | |
| 30 mM | 0.1211 mL | 0.6053 mL | 1.2106 mL | 3.0266 mL | |
| 40 mM | 0.0908 mL | 0.4540 mL | 0.9080 mL | 2.2699 mL | |
| 50 mM | 0.0726 mL | 0.3632 mL | 0.7264 mL | 1.8159 mL | |
| 60 mM | 0.0605 mL | 0.3027 mL | 0.6053 mL | 1.5133 mL | |
| 80 mM | 0.0454 mL | 0.2270 mL | 0.4540 mL | 1.1350 mL | |
| 100 mM | 0.0363 mL | 0.1816 mL | 0.3632 mL | 0.9080 mL |
(R)-(-)-Rolipram Related Classifications
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