AF488 DBCO
Based on 1 publication(s) in Google Scholar
AF488 DBCO is a fluorescent dye and also a click chemistry reagent that can label azide-containing biomolecules. AF488 DBCO mediates the coupling reaction with azide-modified 2'-OMe RNA oligonucleotides. DBCO is a bioorthogonal partner of azides and enables covalent coupling without copper (Ex/Em = 470/520 nm).
For research use only. We do not sell to patients.
- Formula: C48H49N5O11S2
- Molecular Weight:936.06
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) AF488 DBCO
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Biological Activity
Description
In Vitro
AF488 DBCO (100 nmol; 24 h) efficiently conjugates with azido-RNA-S in a 24-hour room temperature reaction, producing a product separable by size exclusion high-performance liquid chromatography[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 936.06
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Formula C48H49N5O11S2
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SMILES
O=C(O)C1=C(C=CC(C(NCCCCCC(N2CC3=C(C#CC4=C2C=CC=C4)C=CC=C3)=O)=O)=C1)C5=C(C6=[O+]C7=C5C=CC(N)=C7S(=O)([O-])=O)C=CC(N)=C6S(=O)(O)=O.CCN(CC)CC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Bioact Mater
Dynamic matrix engineering promotes nascent protein deposition to drive cell migration and expedite Re-epithelization in chronic wound. [Abstract]2025 Oct 28:56:455-467. PMID: 41216373
Solvent & Solubility
In Vitro:
DMSO : 4.68 mg/mL (5.00 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0683 mL | 5.3415 mL | 10.6831 mL | 26.7077 mL |
AF488 DBCO Related Classifications
HY-D2171 Related Classifications
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