AF488 DBCO ditriethylamine
Based on 1 publication(s) in Google Scholar
AF488 DBCO ditriethylamineis a fluorescent dye and also a click chemistry reagent that can label azide-containing biomolecules. AF488 DBCO ditriethylaminemediates the coupling reaction with azide-modified 2'-OMe RNA oligonucleotides. DBCO is a bioorthogonal partner of azides and enables covalent coupling without copper (Ex/Em = 470/520 nm).
For research use only. We do not sell to patients.
- Purity : 95.76%
- Formula: C54H64N6O11S2
- Molecular Weight:1037.25
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) AF488 DBCO ditriethylamine
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Biological Activity
Description
In Vitro
AF488 DBCO ditriethylamine (100 nmol; 24 h RT) efficiently forms a conjugate with azido-RNA-S via click chemistry at room temperature over 24 h, as confirmed by SE-HPLC purification of a single main product peak[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Appearance Solid
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Molecular Weight 1037.25
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Formula C54H64N6O11S2
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Color Orange to red
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SMILES
O=C(O)C1=C(C=CC(C(NCCCCCC(N2CC3=C(C#CC4=C2C=CC=C4)C=CC=C3)=O)=O)=C1)C5=C(C6=[O+]C7=C5C=CC(N)=C7S(=O)([O-])=O)C=CC(N)=C6S(=O)(O)=O.CCN(CC)CC.CCN(CC)CC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Bioact Mater
Dynamic matrix engineering promotes nascent protein deposition to drive cell migration and expedite Re-epithelization in chronic wound. [Abstract]2025 Oct 28:56:455-467. PMID: 41216373
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AF488 DBCO ditriethylamine Related Classifications
HY-D2171A Related Classifications
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