2. Others NF-κB MAPK/ERK Pathway Stem Cell/Wnt Apoptosis Immunology/Inflammation
  3. Drug Derivative NF-κB JNK ERK p38 MAPK TNF Receptor Interleukin Related
  4. Anti-inflammatory agent 99

Anti-inflammatory agent 99 is a chalcone derivative. Anti-inflammatory agent 99 inhibits LPS (HY-D1056)-induced NF-κB nuclear translocation and suppress the phosphorylation of JNK, ERK, and p38. Anti-inflammatory agent 99 inhibits the expression of cytoinflammatory factors such as TNF-α and IL-6. Anti-inflammatory agent 99 can be used for the research of LPS-induced septic shock.

For research use only. We do not sell to patients.

Anti-inflammatory agent 99

Anti-inflammatory agent 99 Chemical Structure

CAS No. : 1345412-48-7

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Anti-inflammatory agent 99 Related Antibodies

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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Description

Anti-inflammatory agent 99 is a chalcone derivative. Anti-inflammatory agent 99 inhibits LPS (HY-D1056)-induced NF-κB nuclear translocation and suppress the phosphorylation of JNK, ERK, and p38. Anti-inflammatory agent 99 inhibits the expression of cytoinflammatory factors such as TNF-α and IL-6. Anti-inflammatory agent 99 can be used for the research of LPS-induced septic shock[1].

IC50 & Target[1]

JNK

 

NF-κB

 

p38

 

ERK

 

IL-6

 

In Vitro

Anti-inflammatory agent 99 (Compound 26) (2.5-10 μM; 24 h) dose-dependently inhibits LPS-induced TNF-α and IL-6 release in mouse RAW 264.7 macrophages, with IC50 values of 8.42 μM and 5.55 μM, respectively[1].
Anti-inflammatory agent 99 (10 μM; 8 h) inhibits LPS-induced TNF-α, IL-6, IL-1β, and IL-12 mRNA expression in mouse primary peritoneal macrophages, but does not significantly reduce COX-2 mRNA levels[1].
Anti-inflammatory agent 99 (2.5-20 μM; 2.5-3 h) inhibits LPS-induced NF-κB p65 nuclear translocation, and dose-dependently prevents LPS-induced IκB degradation in mouse primary peritoneal macrophages[1].
Anti-inflammatory agent 99 (2.5-20 μM; 2.5 h) dose-dependently inhibits LPS-induced JNK phosphorylation in mouse primary peritoneal macrophages[1].
Anti-inflammatory agent 99 (2.5-20 μM; 3 h) inhibits LPS-induced ERK and p38 phosphorylation, and dose-dependently blocks LPS-induced p38 and ERK activation in mouse primary peritoneal macrophages[1].
Anti-inflammatory agent 99 (10 μM; 5 h) inhibits high Glucose (HY-B0389)-induced TNF-α, IL-1β, IL-6, and IL-12 mRNA expression in mouse primary peritoneal macrophages[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-inflammatory agent 99 Related Antibodies

ELISA Assay[1]

Cell Line: mouse RAW 264.7 macrophages
Concentration: 2.5 μM; 5.0 μM; 10 μM
Incubation Time: 24 h
Result: Dose-dependently inhibited LPS-induced TNF-α and IL-6 release.
Exhibited an IC50 of 8.42 μM for TNF-α inhibition and an IC50 of 5.55 μM for IL-6 inhibition.
Significantly reduced TNF-α and IL-6 levels compared to the LPS-only group at 10 μM (p < 0.01).

Real Time qPCR[1]

Cell Line: mouse primary peritoneal macrophages
Concentration: 10 μM
Incubation Time: 8 h
Result: Inhibits LPS-induced TNF-α, IL-6, IL-1β, and IL-12 mRNA expression.
Did not significantly reduce COX-2 mRNA levels.

Immunofluorescence[1]

Cell Line: mouse primary peritoneal macrophages
Concentration: 10 μM
Incubation Time: 2.5 h
Result: Inhibited LPS-induced NF-κB p65 nuclear translocation.

Western Blot Analysis[1]

Cell Line: mouse primary peritoneal macrophages
Concentration: 2.5 μM; 5 μM; 10 μM; 20 μM
Incubation Time: 3 h
Result: Dose-dependently inhibited LPS-induced IκB degradation, JNK phosphorylation and activation of p38 and ERK.
Inhibited LPS-induced ERK and p38 phosphorylation at 10 μM.

Real Time qPCR[1]

Cell Line: mouse primary peritoneal macrophages
Concentration: 10 μM
Incubation Time: 5 h
Result: Inhibited high Glucose-induced TNF-α, IL-1β, IL-6, and IL-12 mRNA expression.
In Vivo

Anti-inflammatory agent 99 (Compound 26) (15 mg/kg; i.v.; single dose) confers an 80% survival rate in mice with LPS-induced inflammatory shock, significantly reducing LPS-induced lethality[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (B6) (male, 18−22 g, LPS-induced inflammatory shock model)[1]
Dosage: 15 mg/kg
Administration: i.v.; single dose
Result: Exhibited an 80% survival rate over the 7-day observation period.
Showed body weight decrease during the first 60 hours after LPS treatment that regained slowly afterward.
Molecular Weight

274.26

Formula

C16H12F2O2
CAS No.
SMILES

O=C(C1=CC=C(C=C1)OC)/C=C/C2=C(C=CC=C2F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-inflammatory agent 99 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-inflammatory agent 991345412-48-7Drug DerivativeNF-κBJNKERKp38 MAPKTNF ReceptorInterleukin RelatedDrug derivativeNuclear factor-κBNuclear factor-kappaBc-Jun N-terminal kinaseExtracellular signal regulated kinasesTumor Necrosis Factor ReceptorTNFRILmouse primary peritoneal macrophagesmacrophagesmouse RAW 264.7 macrophagesTNF-αp38LPSIL-6Inhibitorinhibitorinhibit

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