Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410)
Based on 1 Customer Validation
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) is an anti-mouse/rat/human CD47/IAP IgG1 monoclonal antibody. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can effectively block CD47 signaling and enhance macrophage phagocytic function. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can increase the infiltration of immune cells. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) may interfere with wound healing. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can be used for researches on cancer and inflammation conditions such as breast cancer and intestinal mucosal repair.
For research use only. We do not sell to patients.
- Purity : 99.57%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
Mouse IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human/Mouse/Rat
IC50 & Target
CD47/IAP
In Vitro
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (10 μg/mL, 24 h) significantly inhibits epithelial cell migration and wound closure[1].
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (10-20 μg/mL, 2-4 h) significantly enhances the phagocytic activity of macrophages towards tumor cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Epithelial cells
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Concentration:10 μg/mL
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Incubation Time:24 h
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Result:Significant delayed in cell migration and wound closure.
In Vivo
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (400 μg, intratumorally injection, once every 2 days, for 30 days) significantly inhibits tumor growth in FVB mice bearing MT1A2 tumors[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57Bl/6J mice with a biopsy-based mucosal wound (8-12 weeks)[1]
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Dosage:400 μg
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Administration:Intraperitoneal injection (i.p.), single dose, 24 hours after the wound
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Result:Caused delayed wound closure.
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Animal Model:5×104 MT1A2 cells injected FVB mice (6-8 weeks)[2]
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Dosage:400 μg
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Administration:Intratumorally injection, once every 2 days, for 30 days
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Result:Significantly reduced tumor weight.
Caused a large infiltration of inflammatory cells around the tumor.
Did not cause significant toxicity.
Gene ID
Accession
Q61735-1
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay, Research in vivo
Verified Bioactivity
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Immobilized CD47 Protein, Mouse (HEK293, solution, HY-P74289) can bind Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410). The EC50 for this effect is 179.7 ng/mL. -
Flow cytometric analysis of 106 Jurkat cells with Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (HY-P990132, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Reed M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019 Nov 1;10(1):5004. [Content Brief]
[2]. Willingham SB, et al. The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6662-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)