Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) is an anti-mouse/rat/human CD47/IAP IgG1 monoclonal antibody. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can effectively block CD47 signaling and enhance macrophage phagocytic function. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can increase the infiltration of immune cells. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) may interfere with wound healing. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can be used for researches on cancer and inflammation conditions such as breast cancer and intestinal mucosal repair^[1][2].

Mouse IgG1 kappa

Mouse IgG1 kappa, Isotype Control

Human/Mouse/Rat

CD47/IAP

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (10 μg/mL, 24 h) significantly inhibits epithelial cell migration and wound closure^[1].
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (10-20 μg/mL, 2-4 h) significantly enhances the phagocytic activity of macrophages towards tumor cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (400 μg, i.p., single dose, 24 hours after the wound) significantly inhibits mucosal wound healing in male C57Bl/6J mice with a biopsy-based mucosal wound model^[1].
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (400 μg, intratumorally injection, once every 2 days, for 30 days) significantly inhibits tumor growth in FVB mice bearing MT1A2 tumors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

16423  [NCBI]

Q61735-1

ELISA, FACS, Functional assay, Research in vivo

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Immobilized CD47 Protein, Mouse (HEK293, solution, HY-P74289) can bind Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410). The EC₅₀ for this effect is 179.7 ng/mL.
• 
  Flow cytometric analysis of 10⁶ Jurkat cells with Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (HY-P990132, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Reed M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019 Nov 1;10(1):5004.  [Content Brief]

  [2]. Willingham SB, et al. The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6662-7.  [Content Brief]