2B4 Antibody (YA4476)
(Synonyms: 2B4; NAIL; Nmrk; NKR2B4; SLAMF4)2B4 Antibody (YA4476) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to 2B4.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:50-1:200 | 1:200-1:400 | 1:10000 |
Product Details
2B4 Antibody (YA4476) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to 2B4.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 42 kDa
Purified recombinant fragment of human CD244 (AA: 22-229) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
2B4 is a Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN. Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs)[1][2][3][4][5][6][7].
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Subcellular Localization
Membrane; Single-pass type I membrane protein; Cell membrane; Membrane raft
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Expression
Tissue_specificity:It is expressed in the spleen and peripheral blood lymphocytes, followed by the lungs, liver, testes, and small intestine. It is also expressed on all natural killer (NK) cells, monocytes and basophils, TCR-γ/δ+ T cells, monocytes, basophils, and some CD8 (+) T cells. -
Isoforms & Post-Translational Modification
Q9BZW8 has 4 isomers: Q9BZW8-1: 41616 Da (predicted); Q9BZW8-2: 41077 Da (predicted); Q9BZW8-3: 36987 Da (predicted); Q9BZW8-4: 30784 Da (predicted).
N-linked glycosylation is essential for the binding to its ligand CD48 (PubMed:21606496). Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding (PubMed:21606496);Phosphorylated by FYN and CSK on tyrosine residues following activation (PubMed:11034353). Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells (PubMed:10358138) -
Subunit
Interacts with CD48 (PubMed:27249817, PubMed:9841922). Interacts (via phosphorylated ITSM 1-4) with SH2D1A (via SH2 domain); SH2D1A probably mediates association with FYN. Interacts (via phosphorylated ITSM 3) with PTPN11/SHP-2, INPP5D/SHIP1, PTPN6/SHP-1 and CSK; binding of SH2D1A/SAP prevents association with PTPN11, PTPN6 and CSK; conflictingly a similar association has been described for phosphorylated ITSM 1 also including GRB2 and PLCG1. Interacts weakly (via phosphorylated ITSM 2) with PTPN11/SHP-2 and CSK (PubMed:10358138, PubMed:10934222, PubMed:12458214, PubMed:15713798, PubMed:24642916, PubMed:26221972). Interacts with SH2D1B (PubMed:12458214, PubMed:24642916). Interacts with PIK3R1; PI3K recruits SH2D1A (PubMed:11815622). Interacts with MHC class I proteins; the interaction is proposed to prevent self-killing of NK cells
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SwissProt ID
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Synonyms
2B4; NAIL; Nmrk; NKR2B4; SLAMF4
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